Experiment device for inducing mouse cardiopulmonary vascular diseases

Abstract

An experiment device for inducing mouse cardiopulmonary vascular diseases is mainly used for building common cigarette induced mouse cardiopulmonary vascular disease models. The experiment device comprises a smoking box, a support, mouse bins and a draught fan. The smoking box is bonded on the T-shaped support, the draught fan is installed at the left end of the smoking box, a smoke concentration induction probe is arranged on the smoking box and on the right side of the draught fan, a gas inlet plate is bonded at the right end of the smoking box, a dispersing plate is bonded in the smoking box and on the left side of the gas inlet plate, and 10-12 tapered round holes are formed in the middle of the smoking box in a front-back symmetrical mode. One mouse bin is inserted into each tapered round hole. The experiment device has the advantages that the structure is simple, usage is convenient, and the smoke concentration is adjustable and distributed evenly; mice only smoke through mouths and noses, and other parts of bodies of the mice are not in the smoke, so that the smoking mode of the mice is closer to a human smoking mode, reliability of experiment data is greatly improved, and differences of experiment results are decreased.

Description

technical field [0001] The invention relates to a medical experiment apparatus, in particular to an experimental device for inducing cardiopulmonary and vascular diseases in mice, which is mainly used for establishing a model of cardiopulmonary and vascular diseases in mice induced by ordinary cigarettes. Background technique [0002] Cardiopulmonary vascular disease is a highly disabling and fatal disease, which is closely related to aortic plaque rupture, platelet activation, coagulation and fibrinolytic dysfunction leading to arterial thrombosis. Smoking is one of the most important risk factors for cardiovascular death. Case reports of sudden death from cardiopulmonary and vascular diseases in healthy smokers in a short period of time can be seen in clinical practice. At present, there is no effective preventive measure for smoking-induced cardiopulmonary vascular disease, but the fundamental measure is to quit smoking. Scientific research has shown that smoking can tr...

AI Technical Summary

Problems solved by technology

In order to establish a smoking mouse cardiopulmonary vascular disease model, it is necessary to use a mouse smoking experimental device. At present, the smoking experimental device used in the smoking mouse cardiopulmonary vascular disease model is roughly divided into two types. The first experimental device includes an air pump , constant pressure valve, constant flow valve, flue gas collector, flue gas mixer, smoking box, this kind of smoking experimental device has a complex structure, and the smoke concentration finally sent to the smoking box is less than 3%, which will increase the test period. And the difference between the test data between the experimental group and the control group is not significant, which affects the test results; the second experimental device is only composed of a smoking box and an air pump. There is a cigarette socket on the top of the smoking box. The lower part of the smoking box is connected to a small exhaust fan. The disadvantage of this experimental device is that the smoke concentration and wind speed in the smoking box are not uniform, which will cause some mice to smoke more, and some mice to smoke less. The results are quite different

Another common shortcoming of the above two experimental devices is that the design of the smoking box is unreasonable. During the experiment, several mice are put into the smoking box together. When the mice smell the smoke, they will shrink their bodies and crowd into each other. , hide your nostrils in the fur, which greatly affects the effect of actual nostril smoking, making nostril smoking into fur smoking, making the experimental data inaccurate and unreliable

Furthermore, because the position of each mouse in the smoking box is different, the actual amount of smoking is also different, which also increases the variability of the test results.

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