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A kind of microorganism immobilized loofah and its preparation method and application

A technology of microorganism immobilization and loofah, applied in chemical instruments and methods, immobilized on or in inorganic carriers, biological water/sewage treatment, etc., can solve the problem of weak combination of microorganisms and inorganic carriers and insufficient immobilization effect , The environment is easy to cause harm and other problems, and achieves the effect of excellent hydrophilicity, high SS adhesion, and no harm to the environment

Inactive Publication Date: 2018-07-10
SHANGHAI JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Inorganic carriers include porous glass, diatomaceous earth, activated carbon, quartz sand, etc. Inorganic carriers are often used in adsorption methods. One of the indicators of high-quality inorganic carriers is that they have a large surface area, but the combination of microorganisms and inorganic carriers in the adsorption method is not firm. It is easy to fall off, and the amount of adsorption is small; natural polymer carriers include agar, calcium alginate, carrageenan, etc., which are non-biologically toxic and have good mass transfer properties, but their strength is low, and they are easily biodegraded under anaerobic conditions; Synthetic organic polymer carriers include ACAM, PVA, polyacetyl chitobutyl ester, photosensitive polyvinyl alcohol, etc. Generally, the strength is good, but the mass transfer performance is poor, and the cell activity is affected after embedding.
[0004] To sum up, these existing carrier materials are used as microorganism immobilization carriers. On the one hand, the immobilization effect is not good enough, and they are easy to be lost and inactivated. On the other hand, the production and use of artificial carriers are easy to cause harm to the environment. Environmental friendly
Therefore, choosing a suitable carrier to immobilize microorganisms is particularly important in the field of industrial wastewater and domestic sewage treatment at this stage, and in the field of in-situ remediation technology for groundwater pollution: microbial permeable reaction wall technology, microorganisms immobilized in porous grid frame structure carriers is this The key to the application of a technology, no one has used this technology yet

Method used

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  • A kind of microorganism immobilized loofah and its preparation method and application
  • A kind of microorganism immobilized loofah and its preparation method and application
  • A kind of microorganism immobilized loofah and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] (1) Preparation of anaerobic granular sludge:

[0034] The aerobic sludge retrieved from the water purification plant is placed indoors in a closed state for 3 days, the supernatant is poured off, 1500mL of the bottom sludge is removed, and the bottom sludge is diluted with 500mL of fresh nutrient solution to make a microbial suspension Put it into an Erlenmeyer flask, plug the mouth of the bottle with a rubber stopper, and seal it with a parafilm, and insert a syringe needle on the rubber sieve for exhaust, and acclimate and cultivate it in a constant temperature incubator at 30°C to form anaerobic granular sludge .

[0035] The domestication and cultivation process of bacteria:

[0036] The acclimatization of microorganisms was carried out in an Erlenmeyer flask of about 2200mL, which contained 2000mL of microbial suspension and 200mL of headspace. A syringe needle is provided for exhausting, and placed in a constant temperature incubator at 30°C for cultivation. T...

Embodiment 2

[0043] Embodiment 2: comparative test:

[0044] (1) For high-density natural loofah, ordinary natural loofah, AQUAPOROUSGEL (commercial polymer carrier) and polyurethane sponge, the water absorption, structure and SS adhesion are compared, as shown in Table 1 below:

[0045] Table 1: Comparison table of water absorption, structure and SS adhesion of various carriers

[0046]

[0047] *:H 2 O-g dry carrier-g -1

[0048] Dehydration conditions: 100rpm, 5min

[0049] AQUAPOROUSGEL: A mature polymer commercial carrier (Japan)

[0050] It can be seen from Table 1 that the high-density natural loofah has the highest water retention all the time, and it is higher than the mature commercial carrier AQUAPOROUSGEL; at the same time, the high-density natural loofah has a dense , Complex staggered network structure, has the largest SS adhesion.

[0051] (2) Select AQUAPOROUSGEL, high-density natural loofah, common loofah and polyurethane sponge, 0.32g each of four carriers, and p...

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Abstract

The invention belongs to the technical field of water treatment, and particularly relates to microbial immobilization loofah sponge as well as a preparation method and application thereof. The preparation method comprises the following steps: leaving aerobic sludge as seeding sludge to stand under a closed state, precipitating, taking sediment, adding a nutrient solution for dilution to obtain a microbial suspension, and performing acclimation and culture under the constant temperature of 30 DEG C to form anaerobic granular sludge; drying cleaned high-density and natural loofah sponge for 22-25 hours at 48-52 DEG C, cooling, and performing drying preservation to prepare a loofah sponge vector for later use; mixing the prepared anaerobic granular sludge with the nutrient solution to form a mixed solution, soaking the prepared loofah sponge vector in the mixed solution, and performing closed culture at the constant temperature of 30 DEG C to obtain the microbial immobilization loofah sponge. The microbial immobilization loofah sponge is small in wear coefficient, excellent in mechanical abrasion resistance and long in service life, can reduce the use cost, and is applied to wastewater treatment, very good in removing effect on organic matters, high in wastewater treatment efficiency and free of damage to environment.

Description

technical field [0001] The invention belongs to water treatment technology, in particular to a microorganism-immobilized loofah and its preparation method and application. Background technique [0002] Immobilized microorganism technology is a method of positioning free cells or enzymes in a limited area by chemical or physical means to keep them active and reusable. Immobilized microorganism technology overcomes the shortcomings of too small biological cells, difficult separation from aqueous solution, and easy to cause secondary pollution, and maintains the advantages of high efficiency, strong stability, and ability to purify and maintain high-efficiency bacteria. It has broad application in the field of wastewater treatment application prospects. [0003] However, in the actual application process, how to immobilize and what kind of carrier to use can keep the immobilized microorganisms at a certain strength and activity for a long time, reduce the cost of immobilizatio...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N11/14C02F3/34
CPCC02F3/34C02F2305/06C12N11/14
Inventor 王文兵仵彦卿张旭
Owner SHANGHAI JIAOTONG UNIV
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