Epstein-Barr virus typing kit based on mutation site detection

Abstract

The invention relates to an application of a composition of variable sites selected from an EB virus genome to preparing of an EB virus typing kit, and the EB virus typing kit based on detection of the variable sites, in particular to an application of a composition of five variable sites selected from an EB virus genome to preparing of an EB virus typing kit. The variable sites include loc613, 1oc632, loc7048, loc44531 and 1oc146628. The EB virus typing kit based on detection of the variable sites comprises a PCR amplification primer and a single-base extension primer of the composition of the five variable sites of the EB virus genome, and the variable sites include loc613, 1oc632, loc7048, loc44531 and 1oc146628. According to the EB virus typing kit, an EB virus subtype classification standard can be built, and the relative risk degree for individuals to suffer from nasopharyngeal carcinoma is estimated; the EB virus typing kit is suitable for screening of people in a high nasopharyngeal-carcinoma incidence area, and has the positive guiding significance and the important application value for tumor preventing, tumor early diagnosing and early curing and prognosis improving.

Description

technical field [0001] The invention belongs to the field of PCR detection kits, and relates to the use of a combination of mutation sites selected from the Epstein-Barr virus genome in the preparation of an Epstein-Barr virus typing kit, and an EB virus typing kit based on the detection of these mutation sites. Background technique [0002] With the prolongation of human life expectancy and changes in life behavior, malignant tumors have become an important disease that seriously threatens human health and a major global public health problem. In recent years, the incidence of malignant tumors in China has been high, and the annual new cases account for more than 20% of the new cases in the world. According to statistics, there are about 3.5 million new cancer cases in China every year, and more than 2 million deaths. cause of death. Cancer can be reduced and controlled through the implementation of evidence-based strategies for cancer prevention, early detection, and mana...

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Problems solved by technology

[0005] At present, there is no effective Epstein-Barr virus vaccine for nasopharyngeal carcinoma. The identification of Epstein-Barr virus mutations and subtypes related to nasopharyngeal carcinoma is very important for the development and design of vaccines. provide important reference

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