Joint evaluation model for human-body bioavailability and toxicity of lead in food

A technology for evaluating models and utilization rates, applied in the determination/inspection of microorganisms, methods of supporting/immobilizing microorganisms, artificial cell constructs, etc., which can solve the differences in lead bioavailability, lack of lead evaluation, lack of mucus secretion functions, etc. problem, to achieve the effect of sensitive and efficient evaluation

Inactive Publication Date: 2016-05-11
GUANGXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But the model has flaws, notably the lack of mucus secretion
In addition, the model also lacks the evaluation of the transport and accumulation process of lead to organs in the body, which is different from the absorption process of lead in the human body, resulting in differences between the experimental results and the actual bioavailability of lead

Method used

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  • Joint evaluation model for human-body bioavailability and toxicity of lead in food
  • Joint evaluation model for human-body bioavailability and toxicity of lead in food
  • Joint evaluation model for human-body bioavailability and toxicity of lead in food

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Example explanation of the bioavailability and toxicity of lead in rice and mustard greens to the human body according to the present invention

[0042] 1. Test material

[0043] 1.1 Cell lines were purchased from the Shanghai Cell Bank of the Chinese Academy of Sciences.

[0044] 1.2 Main reagents and medicines

[0045] DMEM high glucose complete medium, HBSS, fetal bovine serum were purchased from Gibco Company,

[0046] Trypsin, bile, and pepsin were purchased from Sigma Company,

[0047] Lactate dehydrogenase cytotoxicity detection kit (Nanjing Jiancheng Bioengineering Institute)

[0048] The rest of the reagents were of domestic analytical grade.

[0049] The buffer used is HBSS solution, pH 7.2-7.35.

[0050] 1.4 Main Equipment and Instruments

[0051] Six-well culture plate (Costar, U.S.), Transwell insert cell culture dish (polyester membrane, pore size 0.4 μm, Corning, U.S.), Millicell-ERS transmembrane resistance meter, ultrapure water system (Millipore,...

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Abstract

The invention discloses a joint evaluation model for human-body bioavailability and toxicity of lead in food. The joint evaluation model is characterized by comprising intestinal-mucosa epithelial cells, hepatic cells and a container divided by a microporous membrane into a top side chamber and a substrate side chamber; the intestinal-mucosa epithelial cells are cultured in the top side chamber, and the hepatic cells are cultured in the substrate side chamber. The joint evaluation model is more similar to the human intestinal absorption structure in the model structure, the absorption efficiency of the lead in food in the human body can be accurately measured, the toxicity of the lead in food can be sensitively and efficiently evaluated, and the joint evaluation model can be widely applied to edible safety evaluation of the lead in food.

Description

technical field [0001] The invention relates to a joint evaluation model of bioavailability and toxicity of lead in food to human body. Background technique [0002] In recent years, with the intensification of industrial and agricultural production and human mining, smelting and other activities, a large amount of lead has been discharged into the environment, resulting in lead pollution in the agricultural system. Humans mainly ingest lead through diet and inhalation, and long-term intake of lead-contaminated food can cause lead poisoning in the human body. Food safety evaluation is an important guarantee for the prevention of human lead poisoning. At present, most evaluation methods indirectly calculate the biological effects of lead intake in food on the human body based on the lead content in food, which cannot truly reflect the biological effects of lead intake in food. In addition, conventional in vivo experiments in animals are time-consuming, costly, and have larg...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M3/00C12N5/071C12Q1/02C12Q1/32
CPCC12M25/02C12Q1/02C12Q1/32
Inventor 韦燕燕郑小曼吕梦婷顾明华黎晓峰何冰王学礼
Owner GUANGXI UNIV
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