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Application of tobacco glycosyl transferase gene NtGT4 in regulation of plant cell differentiation

A technology of glycosyltransferase and plant cells, which is applied in the application field of tobacco glycosyltransferase gene NtGT4 in regulating plant cell differentiation, can solve the problems of enzyme biological function reporting, etc. The effect of differentiation level

Active Publication Date: 2016-05-11
GUIZHOU TOBACCO SCI RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Tobacco glycosyltransferase NtGT4 is an enzyme isolated from the tobacco genome, and there is no report on the biological function of the enzyme

Method used

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  • Application of tobacco glycosyl transferase gene NtGT4 in regulation of plant cell differentiation
  • Application of tobacco glycosyl transferase gene NtGT4 in regulation of plant cell differentiation
  • Application of tobacco glycosyl transferase gene NtGT4 in regulation of plant cell differentiation

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Experimental program
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Embodiment 1

[0034] 1. Materials and methods

[0035] 1. Experimental materials

[0036] 1.1 The cultivated tobacco variety "Guiyan No. 1", Escherichiacoli DH5α, Agrobacterium tumefaciens EHA105, and plant expression vector pBin438 were all preserved by Guizhou Tobacco Research Institute, and the soybean variety "Heinong 63" was provided by Heilongjiang Academy of Agricultural Sciences .

[0037] 1.2 Reagents LATaq enzyme and restriction endonuclease were purchased from TaKaRa Company. DNA gel purification and recovery kit was purchased from Tiangen Biochemical Technology (Beijing) Co., Ltd. T 4 DNA ligase was purchased from NEB Company; pGEM-Tvector vector was purchased from Promega Biotechnology Co., Ltd. ABIPRISTMOptical96-WellReactionPlates, OpticalCaps were purchased from ABI Company; DNA synthesis and sequencing were completed by BGI Technology Services Co., Ltd.

[0038] 2. Experimental method

[0039] 2.1 Primer design

[0040] Design the forward primer (NTGT4-QC-F1) and rev...

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Abstract

The invention relates to an application of tobacco glycosyl transferase gene NtGT4 in the regulation of plant cell differentiation. The novel function of tobacco glycosyl transferase gene NtGT4 is found, the tobacco glycosyl transferase gene NtGT4 can be used to regulate the plant cell differentiation, the differentiation level of plant cells is prominently improved; by comparing the soybean processed by the gene and a contrast, the differentiation of adventitious bud of soybean is prominently improved, and the average differentiation degree can be increased by 40%.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to the application of tobacco glycosyltransferase gene NtGT4 in regulating plant cell differentiation. Background technique [0002] Cell differentiation is an important physiological process in the process of plant growth. The study of the mechanism of plant cell differentiation is helpful to understand the essence of life science. In the genetic transformation of plants, the speed of cell differentiation can be controlled by regulating the differentiation-related genes and direction of differentiation. The main factors known to participate in the differentiation of plant cells are the polarity of plant cells, the relationship between nucleoplasm and cytoplasm, and hormones. Many genes are known to be involved in the process of plant cell differentiation. [0003] Glycosyltransferases are a class of enzymes that link catalytically activated sugars to different receptor mo...

Claims

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Application Information

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IPC IPC(8): C12N15/82A01H5/00
CPCC12N9/1048C12N15/8205C12N15/8229
Inventor 郭玉双任学良余婧余世洲赵杰宏
Owner GUIZHOU TOBACCO SCI RES INST