Method and device for obtaining biophysical characteristics of ultra-diffraction limit cell membrane micro-structure

Abstract

The invention relates to a method and device for obtaining the biophysical characteristics of an ultra-diffraction limit cell membrane micro-structure. The device comprises a laser device and a semiconductor laser device. The laser output path of the laser device sequentially comprises: a polarizer, a first convergent lens set, an aperture, a first reflector, a spatial light modulator, a third convergent lens, a light barrier, a second convergent lest set, and a dichroscope; the reflected light output path of the dichroscope sequentially comprises the objective lens and the objective table of an optical microscope; the transmission light output path of the dichroscope sequentially comprises a filter, a switchable reflector, a sixth convergent lens, and a CCD detector; the laser output path of the semiconductor laser device sequentially comprises a second reflector, a probe, which is driven by a three dimensional controller, and a position sensitive detector; and the CCD detector, the three dimensional controller, and the position sensitive detector are all connected to a data collecting and controlling system. The provided method and device can obtain the information of biophysical characteristics of an ultra-diffraction limit cell membrane micro-structure.

Description

technical field [0001] The invention relates to the coupling of different modal imaging technologies of optical microscopic imaging and atomic force scanning imaging, in particular to a method and device for acquiring biophysical properties of super-diffraction-limited cell membrane microstructures. Background technique [0002] The observation of specific and related biological processes on the cell membrane at the micro-nano scale has become a hot spot in recent years. Ordinary fluorescence optical microscopy occupies an important position in live cell microscopy and detection research due to its non-destructive characteristics to samples. However, since fluorescence microscopy, including laser confocal scanning microscopy, is limited by the diffraction limit, the spatial resolution can only approach half a wavelength. In order to break through the diffraction limit (super-diffraction limit), that is, the spatial resolution is below 200nm, in recent years various super-res...

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Problems solved by technology

However, atomic force probes have no specific recognition ability, while SIM does not have the ability to obtain functional information related to nanoscale ultrastructure.

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