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Rapid detection method of cis-3-hydroxyL-proline in fermentation liquor

A detection method, the technology of hydroxyproline, applied in the direction of color/spectral characteristic measurement, etc., can solve the problems of complex operation, low sensitivity, long reaction time, etc., and achieve cumbersome operation, short detection time, and small sampling volume Effect

Inactive Publication Date: 2016-05-11
JIANGNAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Among them, high-performance liquid chromatography requires expensive instruments and complicated operations, which is not suitable for popularization.
Chloramine T method has low sensitivity, long reaction time, and color reaction with other substances in the fermentation broth

Method used

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  • Rapid detection method of cis-3-hydroxyL-proline in fermentation liquor
  • Rapid detection method of cis-3-hydroxyL-proline in fermentation liquor

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Experimental program
Comparison scheme
Effect test

Embodiment

[0014] 1. This embodiment focuses on the preparation method of the solution and reagent used in the present invention and the flow method of the detection operation.

[0015] 1) Preparation of pretreatment reagents: Accurately draw 1mL of fermentation broth and centrifuge at 9000rpm for two minutes.

[0016] 2) Preparation of CuSO4 solution: Accurately weigh 1.24g of copper sulfate pentahydrate, dilute to 100mL with distilled water, and stir to dissolve.

[0017] 3) Prepare NaOH solution: Accurately weigh 10% sodium hydroxide, dilute to 100mL with distilled water, and stir to dissolve.

[0018] 4) Preparation of H2O2 solution: Accurately draw 2mL of 30% H2O2 solution, dilute to 120mL with distilled water, and stir.

[0019] 5) Preparation of H2SO4 solution: Accurately absorb 39.2g of concentrated sulfuric acid solution, slowly add it into distilled water, dilute to 100mL with distilled water, and stir.

[0020] 6) Preparation of color developer: Accurately weigh 5g of p-dime...

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Abstract

The invention discloses a rapid detection method of cis-3-hydroxyL-proline in fermentation liquor, and aims at providing the rapid detection method of cis-3-hydroxyL-proline, which is convenient to use, simple and fast, and high in flexibility. Compared with a common efficient liquid chromatography or chloramine-T method, the rapid detection method has the advantages that expensive instruments are not needed, operation is easy, the reaction time is short, the number of required samples is small and sensitivity is high. The work efficiency is greatly improved, and wide application and popularization value is achieved.

Description

technical field [0001] The invention belongs to the technical field of spectrophotometry, relates to a detection method, in particular to a rapid detection method of cis-3-hydroxy L-proline in fermentation broth. Background technique [0002] Cis-3-hydroxyl-proline is mainly produced by chemical synthesis and microbial transformation. Among them, the microbial transformation method has the advantages of being non-toxic and harmless, making it the main method for producing cis-3-hydroxy L-proline. At present, the methods for detecting the content of cis-3-hydroxyl-proline in fermentation broth mainly include high performance liquid chromatography and chloramine T method. Among them, high-performance liquid chromatography requires expensive instruments and complicated operations, which is not suitable for popularization. The chloramine T method has low sensitivity, long reaction time, and color reaction with other substances in the fermentation broth. Contents of the inven...

Claims

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Application Information

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IPC IPC(8): G01N21/33
CPCG01N21/33
Inventor 张震宇黄建华王晓姣姚动邦魏照辉
Owner JIANGNAN UNIV
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