Novel function of karounitriol on reducing expression level of DNA transmethylase

Abstract

karounitriol is an important active ingredient from traditional Chinese medicine material semen trichosanthis. The invention discloses a novel function of karounitriol. It is found through experiments of the action of semen trichosanthis glycol on rat primary culture nerve cells that the expression level of DNMT1 genes can be reduced by addition of karounitriol. DNMT1 is the key enzyme causing DNA methylation which is an important constituent part of epigenetics, changes of DNA methylation represent that the nervous system makes a global stress reaction on karounitriol, and it is shown that karounitriol has a significant regulating effect on activities of the nervous system.

Description

technical field

[0001] This application belongs to the technical field of new functions of active ingredients of medicinal materials, and in particular relates to an active ingredient isolated from the Chinese herbal medicine Trichosanthes ziponaris, which can reduce hippocampal neuron DNMT1 New features of gene expression levels. Background technique

[0002] DNA methyltransferase 1, DNMT1, is a key enzyme that catalyzes the methylation of DNA cytosine. DNA methylation is an important modification method for living organisms to sense external input signals and regulate structural gene expression, and is one of the main categories of epigenetics. DNMT1 plays an important role in maintaining DNA methylation characteristics and DNA replication and repair. Under different physiological and pathological backgrounds, the change of DNMT1 expression level indicates the overall change of the target gene, reflecting the overall adaptive or damaging changes of the body.

[0003] Me...

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