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Novel function of karounitriol on reducing expression level of DNA transmethylase

A technology of Trigonella diol and new functions, which is applied in the directions of medical preparations containing active ingredients, steroids, plant raw materials, etc.

Active Publication Date: 2016-05-25
浙江高美生物科技有限公司
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Problems solved by technology

However, the functional effects of the active ingredients of Trichosanthes chinensis, especially Trichosanthene diol, on the central nervous system have not been reported yet.

Method used

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  • Novel function of karounitriol on reducing expression level of DNA transmethylase

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Experimental program
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Effect test

Embodiment 1

[0015] The detection process of the physiological activity of trichosanthene diol is:

[0016] The SD rats born within 24 hours were sacrificed and the hippocampus tissue was taken for primary culture. Put the hippocampus tissue on a 200-mesh sieve, use ophthalmic scissors to cut small pieces and sieve, pour off the supernatant after centrifuging at 1000rpm for 8 minutes, add high-sugar DMEM medium and 10% fetal bovine serum, and plant after pipetting 6-well plate. Culture condition is 37 o C5% level of carbon dioxide. The medium was changed every three days. When cultured to the 7th day, the primary cultured cells were added to the three wells of the primary cultured cells with a final concentration of 50 μM Trichosanthindiol, and the other three wells were added with the same volume of sterile water as a control group. When the culture was continued for 14 days, the cells were recovered. Use conventional techniques in the field to extract total mRNA in cells, reverse tr...

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Abstract

karounitriol is an important active ingredient from traditional Chinese medicine material semen trichosanthis. The invention discloses a novel function of karounitriol. It is found through experiments of the action of semen trichosanthis glycol on rat primary culture nerve cells that the expression level of DNMT1 genes can be reduced by addition of karounitriol. DNMT1 is the key enzyme causing DNA methylation which is an important constituent part of epigenetics, changes of DNA methylation represent that the nervous system makes a global stress reaction on karounitriol, and it is shown that karounitriol has a significant regulating effect on activities of the nervous system.

Description

technical field [0001] This application belongs to the technical field of new functions of active ingredients of medicinal materials, and in particular relates to an active ingredient isolated from the Chinese herbal medicine Trichosanthes ziponaris, which can reduce hippocampal neuron DNMT1 New features of gene expression levels. Background technique [0002] DNA methyltransferase 1, DNMT1, is a key enzyme that catalyzes the methylation of DNA cytosine. DNA methylation is an important modification method for living organisms to sense external input signals and regulate structural gene expression, and is one of the main categories of epigenetics. DNMT1 plays an important role in maintaining DNA methylation characteristics and DNA replication and repair. Under different physiological and pathological backgrounds, the change of DNMT1 expression level indicates the overall change of the target gene, reflecting the overall adaptive or damaging changes of the body. [0003] Me...

Claims

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Application Information

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IPC IPC(8): A61K31/56A61P25/00C07J63/00A61K36/428
CPCA61K31/56C07J63/008
Inventor 徐毅
Owner 浙江高美生物科技有限公司
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