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Latex enhanced immunoturbidimetry kit for content of helicobacter pylori antibodies

A Helicobacter pylori, latex-enhanced technology, which is applied to measurement devices, material analysis, instruments and other directions by observing the impact on chemical indicators, can solve the problems of result impact, long time consumption, manual operation, etc.

Active Publication Date: 2016-05-25
BEIJING STRONG BIOTECH INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Urine, saliva and other secretion antibody detection method, sampling is simple and painless, its sensitivity and specificity are similar to serological tests, but the results may be affected by some factors
[0013] The traditional method of detecting Helicobacter pylori antibody has the disadvantages of low degree of automation, manual operation, and long time-consuming

Method used

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  • Latex enhanced immunoturbidimetry kit for content of helicobacter pylori antibodies
  • Latex enhanced immunoturbidimetry kit for content of helicobacter pylori antibodies
  • Latex enhanced immunoturbidimetry kit for content of helicobacter pylori antibodies

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0094] Example 1: Preparation of Helicobacter pylori Antibody Kit 1

[0095] 1. Preparation of Reagent R1

[0096] In 50mM HEPES buffer at pH 7.2, add 0.9% NaCl by weight / volume, 5% PEG-8000 by weight / volume, 1% BS by weight / volume, 10mM EDTA, 0.1% sodium azide by volume, stir evenly to be R1 reagent.

[0097] 2. Preparation of R2 Reagent

[0098] Polystyrene latex solution (concentration 10% by weight / volume) (purchased from Merck) 0.5mL with a diameter of 150nm, add 4.5mL of 0.05MMES buffer (pH6.0), and then add 0.5mg / ml of cross-linking agent EDAC (purchased from Merck) was activated for 30 minutes;

[0099] Dilute 0.5 mg of Helicobacter pylori antigen (purchased from Calbioreagents) with 5 mL of 0.05 MMES buffer (pH 6.0), immediately add to the above latex solution, and react at 37 ° C for 3 hours;

[0100] Add 1mL of 0.1M glycine buffer (pH7.0) and stir for 1h to stop the reaction, centrifuge to remove the supernatant;

[0101] Wash 3 times with 33 mL of 50 mM glycin...

Embodiment 2

[0106] Example 2: Preparation of Helicobacter pylori Antibody Kit 2

[0107] 1. Preparation of R1

[0108] The preparation method of reagent R1 is the same as in Example 1.

[0109] 2. Preparation of R2 Reagent

[0110] Polystyrene latex solution (concentration 10% by weight / volume) (purchased from Merck) 0.5mL with a diameter of 150nm, add 4.5mL of 0.05MMES buffer (pH6.0); add 0.5mg / ml of cross-linking agent EDAC (purchased from Merck) was activated for 30 minutes;

[0111] After diluting 0.5 mg of Helicobacter pylori antigen containing 0.2% amino-modified PEG-20000 (purchased from Pierce) with 5 mL of 0.05 MMES buffer (pH 6.0), it was immediately added to the above latex solution and reacted at 37 ° C for 3 hours ;

[0112] Add 1mL of 0.1M glycine buffer (pH7.0) and stir for 1h to stop the reaction, centrifuge to remove the supernatant;

[0113] Wash 3 times with 33 mL of 50 mM glycine buffer (pH 8.0), and harvest the latex particles coated with antigen;

[0114] Use 3...

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Abstract

The invention relates to a latex enhanced immunoturbidimetry kit for content of helicobacter pylori antibodies, in particular to a kit for measuring the content of helicobacter pylori antibodies in human serum and plasma samples by using latex enhanced immunoturbidimetry. The kit contains reaction buffer, latex particles crosslinked with helicobacter pylori antigens, and a calibrator. According to the kit, the helicobacter pylori antigens crosslinked with the surfaces of the latex particles and the helicobacter pylori antibodies in a sample are subjected to immunoreaction to form turbidity, and the content of the helicobacter pylori antibodies is detected through the rising degree of the turbidity. The kit can be used for a clinical frequently used biochemical analyzer, and is easy and convenient to operate, speedy and high in specificity. The stability of the kit is improved, and sensitivity and a detection range of the latex enhanced immunoturbidimetry kit can meet requirements of clinical application.

Description

technical field [0001] The disclosure relates to the field of in vitro medical immunodiagnosis; it provides a kit for determining the content of Helicobacter pylori antibody in human serum and plasma samples by using an immunoturbidimetric method. Background technique [0002] Helicobacter pylori (HP) was first reported and discovered in 1983 by Barry J. Marshall and J. Robin Warren. The duo won the 2005 Nobel Prize in Physiology or Medicine. [0003] Helicobacter pylori is a Gram-negative bacterium with unipolar, multi-flagellate, blunt-ended and spirally curved, 2.5-4.0 μm long and 0.5-1.0 μm wide. On the surface of gastric mucosal epithelial cells, it often presents a typical spiral or arc shape. Helicobacter pylori has high nutritional requirements and needs to grow under anaerobic conditions. It is difficult to cultivate in vitro, and the ambient oxygen requirement is 5 to 8%. It cannot grow in the atmosphere or in an absolutely anaerobic environment. [0004] Helico...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569G01N33/574G01N21/82
CPCG01N21/82G01N33/56922G01N33/574G01N33/57446
Inventor 高爱民刘希
Owner BEIJING STRONG BIOTECH INC
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