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Application of C-type natriuretic peptide in preparation of external contraceptive and sperm function detection reagent

A technology for functional detection and detection reagents, applied in the field of medicine and health, can solve problems such as health hazards, and achieve the effects of less semen, no toxic side effects and adverse reactions, and convenient use

Active Publication Date: 2016-06-01
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

If you take it blindly and in large quantities for a long time, it will cause great harm to your health.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1 Application of C-type natriuretic peptide in inducing sperm chemotaxis

[0030] Fresh sperm were taken from the cauda epididymis of adult male mice, and incubated in capacitation solution T6 for 1.5 h for capacitation. The mouse sperm after capacitation was added to the 150 μl T6 droplet in the center of the petri dish, and the sperm density was about 5×10 6 pieces / ml. One end of the capillary (2.5 cm in length, 3 mm in diameter) was closed, and an ampulla was placed at the closed end. The ampulla was derived from the oviduct of a mouse that had just ovulated, and was used as the experimental group. In the control group, the capillary (2.5 cm in length, 3 mm in diameter) was not placed in the ampulla. The open end of the capillary is connected to a droplet of semen. Continuously observe the number of sperm in the capillary in the field of view under the 10x objective lens of the microscope. The experiment was repeated three times.

[0031] It was found th...

Embodiment 2

[0033] Example 2 Application of C-type Natrium Peptide in Sperm Function Detection

[0034] Capabilize the sperm that cannot be naturally fertilized in capacitation solution T6, and then add them to 150 μl of T6 solution microdroplets, with a sperm density of 10 6 pieces / ml. Take a capillary with a length of 2.5 cm and a diameter of 3 mm, with one end closed. Take out the ampulla of the mouse that has just ovulated, put it into the closed end of the capillary, and then connect the open end of the capillary with the seminal fluid droplet. In the control group, the capillary (2.5 cm in length, 3 mm in diameter) did not contain the ampulla. Continuously observe the number of sperm in the field of view under the 10x objective lens of the microscope. At the same time, compare the number of sperm in the capillary of the treatment group and the control group. If there is no significant difference, it indicates that the reason why the semen cannot be naturally fertilized may be NPR2...

Embodiment 3

[0035] Application of Example 3C-type natriuretic peptide in reducing the fertilization rate of mice

[0036] Adult female mice were treated with ovulation induction, injected with a certain concentration of C-type natriuretic peptide into their cervix, and then housed with male mice. The specific method is as follows: 30 adult CD-1 female mice (6-8 weeks), each weighing about 35g, were divided into five groups, six in each group, namely A, B, C, D, E groups, each group The middle female mice are numbered 1, 2, 3, 4, 5, and 6. Five groups of female mice were given different concentrations of C-type natriuretic peptide, respectively, 0, 10×10 -10 , 5×10 -10 、10 -9 、10 -8 mol / l, the injection volume is 20μl. After the adult female mice were induced by gonadotropin (PMSG5IU / only, induced for 48 hours, then HCG5IU / only, induced for 12 hours), inject 20 microliters of C-type natriuretic peptide into the cervix of the female mice with a microinjector, and immediately with adult...

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Abstract

The invention relates to an application of C-type natriuretic peptide in preparation of an external contraceptive and a sperm function detection reagent. According to research findings, at the time of ovulation, the C-type natriuretic peptide is expressed at the ampulla on the fertilization position of the fallopian tube, and the chemotactic movement of sperms toward ovum can be induced to finish fertilization. If the genital tract of a female mammal is added with certain amount of C-type natriuretic peptide or the C-type natriuretic peptide and the sperms are incubated, the concentration gradient distribution of C-type natriuretic peptide in the fallopian tube is changed, and the sperms cannot make chemotactic movement toward ovum along the concentration gradient so as to block fertilization. Based on the sperm chemotaxis principle, the invention provides an external contraceptive taking endogenous protein C-type natriuretic peptide as an active ingredient. The contraceptive does not cause toxic or side effects or adverse reaction, is suitable for contraception of most women of childbearing age and convenient to use and has a broad application prospect. Moreover, the sperm function detection reagent prepared from the C-type natriuretic peptide is adopted for identifying the sperm quality, the operation is simple, the result is easy to observe, a small semen volume is required, and the sperm function detection reagent is suitable for in-vitro detection of sperm function.

Description

technical field [0001] The invention relates to the field of medicine and health, in particular to the application of C-type natriuretic peptide in the preparation of external contraceptives and sperm function detection reagents. Background technique [0002] The number of male infertile populations has increased due to factors such as climate change and faster pace of life and work, and some causes of male infertility have not yet been clarified. Routine semen analysis can mainly understand the information of semen volume, sperm density and total amount of sperm in semen, sperm agglutination, sperm motility and activity rate, sperm morphology, and semen liquefaction. In fact, it is difficult to truly understand the function and fertilization potential of sperm through the above-mentioned routine analysis, especially in artificial insemination and test-tube baby, it is still not possible to decide whether artificial insemination can be performed only through the information ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/17C12Q1/02A61P15/18
CPCA61K38/17A61K38/22C12Q1/02
Inventor 张美佳夏国良孔娜娜张玉李佳
Owner CHINA AGRI UNIV
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