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A Rapid Identification Method of Corydalis Corydalis

A fast, one-to-one technology, applied in biochemical equipment and methods, microbial determination/testing, etc., to achieve strong specificity, good stability, and high sensitivity

Active Publication Date: 2019-06-18
GUANGDONG SECOND TRADITIONAL CHINESE MEDICINE HOSPITAL (GUANGDONG PROVINCE ENG TECH RES INST OF TCM)
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The object of the present invention is to provide a rapid identification method for Corydalis Corydalis, which uses characteristic primers to quickly identify Corydalis Corydalis through PCR technology, and solves the problem of rapid quality control of Corydalis Corydalis medicinal materials in the pharmaceutical process

Method used

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  • A Rapid Identification Method of Corydalis Corydalis
  • A Rapid Identification Method of Corydalis Corydalis
  • A Rapid Identification Method of Corydalis Corydalis

Examples

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Embodiment 2

[0041] Embodiment 2, the present embodiment is to revalidate the method of embodiment 1, collect other samples except the above-mentioned samples, including other counterfeit products (turmeric), processed products of Corydalis Corydalis. Carry out test and durability investigation according to the method for embodiment 1, the results are shown in Figure 4 , Figure 4 Mark in the middle is a 1000bp molecular marker, 1 is a sample of Corydalis decoction pieces, 2 is a sample of turmeric decoction pieces, 3 is a sample of Corydalis decoction pieces stored for 3 years, and 4-7 are samples of Corydalis Corydalis made with vinegar. The DNA in the Corydalis Corydalis decoction pieces that had been placed for 3 years was somewhat degraded, and the resulting bands were also relatively blurred. The experimental results of other samples were consistent with those in Example 1. The results show that the inventive method is suitable for most of the Corydalis identification.

[0042] In...

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Abstract

The invention discloses a quick identifying method of Rhizoma corydalis. Aiming at a pair of feature primers of Rhizoma corydalis, quick identifying is performed on Rhizoma corydalis. The quick identifying method includes following steps and conditions: (1), taking total DNA from collected medicinal materials for testing of each lot; (2), taking the feature primers P2 and P4, and adding sterile water for dilution; (3), obtaining an amplification product through PCR (polymerase chain reaction) process; (4), mixing the amplification product with 6xLoading buffer well, dripping a mixture on agarose gel, disposing the agarose gel below an ultraviolet lamp, observing, photographing and storing; (5), wherein pictures show that genuine Rhizoma corydalis has a clear strip between 200 bp and 300 bp, and other counterfeit products do not have the strip at corresponding positions, and female interference does not exist. The method belongs to quick identifying methods and is based on PCR technology to identify Rhizoma corydalis. The method is simple, accurate, high in sensitivity, low in sampling quantity, high in stability, high in detection speed, high in specificity and quite conducive to being applied in quick quality control of Rhizoma corydalis medicinal materials in the pharmaceutical process.

Description

technical field [0001] The invention relates to the technical field of traditional Chinese medicine quality detection, in particular to a rapid identification method of Corydalis Corydalis. Background technique [0002] Corydalis yanhusuo is the dry tuber of Corydalis yanhusuo W.T.Wang, a plant of the genus Corydalis yanhusuo (Corydalis yanhusuo W.T.Wang). Due to the continuous increase of clinical use, the domestic and foreign market demand continues to increase, resulting in mixed fakes and inferior products, and unscrupulous businessmen shoddy and fake genuine products. [0003] Most of the counterfeit products of Corydalis are from the same genus of plants with similar ingredients, among which whole-leaf Corydalis is the most common substitute and confusion in the raw material market. The scientific identification of varieties and genetic analysis of provenance cannot be carried out only by traditional morphological analysis, and the inspection time is long and the insp...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12Q1/686
CPCC12Q1/686C12Q1/6895C12Q2600/158C12Q2565/125
Inventor 孙冬梅毕晓黎罗文汇胥爱丽陈昭陈伟韬李养学李素梅江洁怡许灿新
Owner GUANGDONG SECOND TRADITIONAL CHINESE MEDICINE HOSPITAL (GUANGDONG PROVINCE ENG TECH RES INST OF TCM)
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