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IL-6 gene rs1524107 site marker associated with chronic hepatitis B virus infections and its application

A chronic hepatitis B, marker technology, applied in the application field of genetic counseling, can solve problems such as affecting liver damage and disease severity, changing expression or binding activity, etc.

Inactive Publication Date: 2016-06-08
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Polymorphisms in this gene may alter its expression or binding activity, thereby affecting liver damage and disease severity

Method used

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  • IL-6 gene rs1524107 site marker associated with chronic hepatitis B virus infections and its application
  • IL-6 gene rs1524107 site marker associated with chronic hepatitis B virus infections and its application
  • IL-6 gene rs1524107 site marker associated with chronic hepatitis B virus infections and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] This embodiment uses a method for detecting the SNP site of the IL-6 gene related to the outcome of chronic hepatitis B.

[0022] 1. Extraction of genomic DNA

[0023] Use the QIAampDNABloodMini kit from Qiagen, USA, and extract DNA from whole blood according to the instructions.

[0024] Preparation before the experiment: transfer the blood sample to the refrigerator at 4°C one day in advance to let the blood melt. Turn on the metal bath and adjust it to 56°C, prepare centrifuge tubes, masks, napkins, kitchen paper, alcohol spray cans, waste paper baskets, waste liquid tanks, etc. (Refer to qiagen kit)

[0025] 1. Add 20ul protease to a 1.5ml centrifuge tube; if RNA needs to be removed, add 4ul RNase to the tube.

[0026] 2. Add 200ul sample to the 1.5ml centrifuge tube; please do not touch the tube wall when drawing blood to avoid contaminating the pipette.

[0027] 3. Add 200ul of buffer AL, fully invert and mix, and shake for 15s.

[0028] Warm bath at 4.56℃ for about 1h.

[0...

Embodiment 2

[0045] This example uses the method of Example 1 to treat 191 patients with chronic hepatitis B, 177 patients with hepatitis B cirrhosis, 48 ​​patients with hepatitis B liver failure, 93 HBsAg and HBeAg negative carriers and 112 patients The genotypes and alleles of IL-6 gene rs1524107 locus of healthy subjects of the International Health Examination Center were analyzed.

[0046] 1. Test object

[0047] This study collected 93 HBsAg and HBeAg-negative carriers and 191 patients with chronic hepatitis B who were hospitalized or outpatients of the First Affiliated Hospital of Zhejiang University School of Medicine from November 2013 to November 2015. There were 177 patients with hepatitis B liver cirrhosis, 48 ​​patients with hepatitis B liver failure, and 112 healthy medical examiners at the International Health Examination Center, totaling 621 cases.

[0048] Patient selection criteria

[0049] Carry out case diagnosis according to the case judgment criteria specified in the 2015 edi...

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Abstract

The invention relates to individual susceptibility of disease progression in a chronic hepatitis B virus (HBV) infected person, in particular to application of an rs1524107 site marker of susceptibility gene IL-6 associated with the disease progression of HBV chronic infected person, in disease outcome warning and genetic counseling for the HBV chronic infected person. The SNP rs1524107 site marker of IL-6 gene associated with the outcome of HBV-related hepatocirrhosis is provided, an rs1524107 site of IL-6 gene is shown in a TT gene type; the progression of the rs1524107 site TT gene type chronic hepatitis B virus is faster, leading to greater proneness to hepatocirrhosis; for a TT gene type chronic hepatitis B virus patient, it is possible for a scientific intervention, and in this way, it is possible to specifically reduce the risk of these susceptible people to suffer from hepatitis b cirrhosis.

Description

Technical field [0001] The present invention relates to the individual susceptibility of chronic hepatitis B virus (HBV) infection to the progression of the disease course. Specifically, the present invention relates to the application of the rs1524107 locus marker of the susceptibility gene IL-6, which is related to the progression of the course of HBV chronic infection, in the early warning of HBV chronic infection and genetic counseling. Background technique [0002] According to data updated by WHO in 2015, there are approximately 240 million people infected with chronic hepatitis B virus (HBV) worldwide. More than 780,000 people worldwide die from various complications of hepatitis B each year, including liver cirrhosis and liver cancer. my country is an area with a high incidence of viral hepatitis. There are about 93 million people with chronic HBV infection, including about 20 million people with chronic hepatitis B (CHB). In general, chronic hepatitis B is a gradual de...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6883C12Q2600/118C12Q2600/156
Inventor 郑敏陈智夏彩霞刘艳宁陈峰黄春红楼国华朱伟
Owner ZHEJIANG UNIV
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