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rs2069852 site marker of chronic hepatitis B virus infection related IL-6 gene and application thereof

A chronic hepatitis B, hepatitis B technology, applied in the application field of genetic counseling, can solve problems such as affecting liver damage and disease severity, changing expression or binding activity, etc.

Active Publication Date: 2016-05-18
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Polymorphisms in this gene may alter its expression or binding activity, thereby affecting liver damage and disease severity

Method used

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  • rs2069852 site marker of chronic hepatitis B virus infection related IL-6 gene and application thereof
  • rs2069852 site marker of chronic hepatitis B virus infection related IL-6 gene and application thereof
  • rs2069852 site marker of chronic hepatitis B virus infection related IL-6 gene and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] This embodiment adopts a method for detecting the SNP site of IL-6 gene related to the outcome of chronic hepatitis B.

[0024] 1. Genomic DNA extraction

[0025] Whole blood DNA was extracted using the QIAampDNABloodMini kit from Qiagen, USA, and according to the instructions.

[0026] Preparation before the experiment: transfer the blood sample to a 4°C refrigerator one day in advance to allow the blood to thaw. Turn on the metal bath and adjust it to 56°C, prepare centrifuge tubes, masks, napkins, kitchen paper, alcohol spray cans, waste paper baskets, waste liquid tanks, etc. (refer to qiagen kit)

[0027] 1. Add 20ul protease to a 1.5ml centrifuge tube; if RNA needs to be removed, add 4ul RNAse to the tube.

[0028] 2. Add 200ul sample to the 1.5ml centrifuge tube; please do not touch the tube wall when drawing blood, so as not to contaminate the pipette.

[0029] 3. Add 200ul buffer solution AL, mix thoroughly by inverting, and shake for 15s.

[0030] 4.56 ℃ ...

Embodiment 2

[0044]This embodiment uses the method of embodiment 1, to 191 routine hepatitis B patients, 177 routine hepatitis B liver cirrhosis patients, 48 ​​routine hepatitis B liver failure patients, 93 routine HBsAg and HBeAg all negative carriers and 112 cases The genotype and allele of the IL-6 gene rs2069852 locus in healthy subjects from the International Health Examination Center were analyzed.

[0045] 1. Test object

[0046] During the period from November 2013 to November 2015, this study collected 93 HBsAg and HBeAg negative carriers, 191 chronic hepatitis B patients, 177 cases of hepatitis B cirrhosis patients, 48 ​​cases of hepatitis B liver failure patients and 112 healthy checkups from the International Health Examination Center, a total of 621 cases.

[0047] Patient Inclusion Criteria

[0048] Case diagnosis was carried out according to the case judgment criteria stipulated in the 2015 edition of "Guidelines for the Prevention and Treatment of Chronic Hepatitis B":

...

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Abstract

The invention relates to individual susceptibility of the disease progression of chronic hepatitis B virus (HBV) infected people. Specifically, the invention relates to an application of an rs2069852 site marker of a susceptibility gene IL-6 related to the disease progression of HBV chronic infected people in the early warning of disease outcome of HBV chronic infected people and genetic counseling. The invention provides an rs2069852 site marker of the IL-6 gene related to the outcome of chronic hepatitis B and particularly the outcome of hepatitis B cirrhosis as well as a detection reagent and detection method thereof, and also provides an rs2069852 site marker of the IL-6 gene and an application thereof in the early warning of chronic HBV infected people. The hepatitis B carrier with AG genotype can be scientifically intervened, for example, by taking measures such as therapeutic prevention as early as possible; and therefore, the risk of hepatitis B cirrhosis of the susceptible people can be reduced in a targeted manner.

Description

technical field [0001] The present invention relates to the individual susceptibility of chronic hepatitis B virus (HepatitisBvirus, HBV) infection to progress of disease course. Specifically, the present invention relates to the application of the rs2069852 site marker of the susceptibility gene IL-6 related to the progression of HBV chronic infection in the early warning of HBV chronic infection and genetic counseling. Background technique [0002] According to the updated data of WHO in 2015, there are about 240 million people with chronic hepatitis B virus (HBV) infection worldwide. Worldwide, more than 780,000 people die from various complications of hepatitis B every year, including liver cirrhosis and liver cancer. my country is a high-incidence area of ​​viral hepatitis. There are about 93 million chronic HBV infected people, including about 20 million chronic hepatitis B (CHB) patients. Generally, chronic hepatitis B develops gradually and causes complications, bu...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6883C12Q2600/118C12Q2600/156
Inventor 郑敏陈智夏彩霞刘艳宁陈峰黄春红楼国华朱伟
Owner ZHEJIANG UNIV
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