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IL-6 Gene rs2069852 Locus Marker Related to Chronic Hepatitis B Virus Infection and Its Application

A technology of IL-6, hepatitis B, applied in the application field of genetic counseling, can solve problems affecting liver injury and disease severity, changing expression or binding activity, etc.

Active Publication Date: 2019-02-26
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Polymorphisms in this gene may alter its expression or binding activity, thereby affecting liver damage and disease severity

Method used

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  • IL-6 Gene rs2069852 Locus Marker Related to Chronic Hepatitis B Virus Infection and Its Application
  • IL-6 Gene rs2069852 Locus Marker Related to Chronic Hepatitis B Virus Infection and Its Application
  • IL-6 Gene rs2069852 Locus Marker Related to Chronic Hepatitis B Virus Infection and Its Application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] In this example, a method for detecting IL-6 gene SNP loci related to the prognosis of chronic hepatitis B is adopted.

[0024] 1. Extraction of genomic DNA

[0025] Whole blood DNA was extracted using the QIAamp DNA Blood Mini kit from Qiagen, USA, according to the instructions.

[0026] Preparation before the experiment: transfer the blood samples to a 4°C refrigerator one day in advance to thaw the blood. Open the metal bath and adjust to 56°C, prepare centrifuge tubes, masks, napkins, kitchen paper, alcohol watering can, waste paper basket, waste tank, etc. (refer to qiagen kit)

[0027] 1. Add 20ul of protease to a 1.5ml centrifuge tube; add 4ul of RNase to the tube if RNA needs to be removed.

[0028] 2. Add 200ul sample to the 1.5ml centrifuge tube; please do not touch the tube wall when drawing blood to avoid contamination of the pipette.

[0029] 3. Add 200ul buffer AL, invert and mix well, and shake for 15s.

[0030] 4. Incubate at 56℃ for about 1h.

[0...

Embodiment 2

[0044]This embodiment uses the method of embodiment 1, to 191 routine hepatitis B patients, 177 routine hepatitis B liver cirrhosis patients, 48 ​​routine hepatitis B liver failure patients, 93 routine HBsAg and HBeAg all negative carriers and 112 cases The genotype and allele of the IL-6 gene rs2069852 locus in healthy subjects from the International Health Examination Center were analyzed.

[0045] 1. Test object

[0046] During the period from November 2013 to November 2015, this study collected 93 HBsAg and HBeAg negative carriers, 191 chronic hepatitis B patients, 177 cases of hepatitis B cirrhosis patients, 48 ​​cases of hepatitis B liver failure patients and 112 healthy checkups from the International Health Examination Center, a total of 621 cases.

[0047] Patient Inclusion Criteria

[0048] Case diagnosis was carried out according to the case judgment criteria stipulated in the 2015 edition of "Guidelines for the Prevention and Treatment of Chronic Hepatitis B":

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Abstract

The invention relates to individual susceptibility of the disease progression of chronic hepatitis B virus (HBV) infected people. Specifically, the invention relates to an application of an rs2069852 site marker of a susceptibility gene IL-6 related to the disease progression of HBV chronic infected people in the early warning of disease outcome of HBV chronic infected people and genetic counseling. The invention provides an rs2069852 site marker of the IL-6 gene related to the outcome of chronic hepatitis B and particularly the outcome of hepatitis B cirrhosis as well as a detection reagent and detection method thereof, and also provides an rs2069852 site marker of the IL-6 gene and an application thereof in the early warning of chronic HBV infected people. The hepatitis B carrier with AG genotype can be scientifically intervened, for example, by taking measures such as therapeutic prevention as early as possible; and therefore, the risk of hepatitis B cirrhosis of the susceptible people can be reduced in a targeted manner.

Description

technical field [0001] The present invention relates to the individual susceptibility of chronic hepatitis B virus (Hepatitis B virus, HBV) infection to progression of disease course. Specifically, the present invention relates to the application of the rs2069852 locus marker of the susceptibility gene IL-6, which is related to the progression of HBV chronic infection, in the early warning and genetic counseling of HBV chronic infection. Background technique [0002] According to data updated by WHO in 2015, there are approximately 240 million people with chronic hepatitis B virus (HBV) infection worldwide. Worldwide, more than 780,000 people die each year from various complications of hepatitis B, including cirrhosis and liver cancer. my country is a high incidence area of ​​viral hepatitis. There are about 93 million people with chronic HBV infection, including about 20 million people with chronic hepatitis B (CHB). Generally, chronic hepatitis B develops gradually and c...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6883C12N15/11
CPCC12Q1/6883C12Q2600/118C12Q2600/156
Inventor 郑敏陈智夏彩霞刘艳宁陈峰黄春红楼国华朱伟
Owner ZHEJIANG UNIV
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