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Biological control method and biocontrol agent for banana vasicular wilt

A technology of banana fusarium wilt and biological control preparations, applied in the direction of microorganism-based methods, biochemical equipment and methods, biocides, etc., can solve problems affecting banana quality, general effect, and polluting the environment

Inactive Publication Date: 2016-06-15
昆明保腾生化技术有限公司 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chemical pesticide control is not only ineffective, but also pollutes the environment and affects the quality of bananas; most disease-resistant varieties can only resist a single physiological race of banana fusarium wilt, and there is a danger of large-scale outbreaks when other physiological races invade

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0078] After the banana tissue-cultured seedlings were transplanted and survived, spray No. 1, No. 2 and No. 3 200 times of liquid or 500 times of liquid or 1000 times of liquid of compound microbial inoculum every 10 days. Three times, the untreated banana seedlings were used as blank control and transplanted to the same field as the treated banana seedlings, and managed according to conventional methods. 30 days, 60 days, 90 days, and 120 days after transplanting, the disease of banana wilt disease was investigated respectively. The survey results showed that the incidence of banana wilt disease was higher than that of the control group 30 days, 60 days, 90 days, and 120 days after transplanting. Decreased by 75.45-86.15%, 70.66-79.71%, 67.48-76.27%, 56.81-68.16%.

Embodiment 2

[0080] When transplanting banana tissue-cultured seedlings, mix No. 1, No. 2 and No. 3 composite microbial agents into the transplanting matrix or soil of the tissue-cultured seedlings respectively, and each cubic seedling of the tissue-cultured transplanting matrix Or mix 0.5kg or 2.5kg or 5kg compound microbial bacterial agent in the soil, transplant the untreated banana seedlings to the same field as the blank control and the banana seedlings of the treatment group, and manage them according to conventional methods. 30 days, 60 days, 90 days, and 120 days after transplanting, the disease of banana wilt disease was investigated respectively. The survey results showed that the incidence of banana wilt disease was higher than that of the control group 30 days, 60 days, 90 days, and 120 days after transplanting. Decreased by 72.18-83.62%, 68.75-77.19%, 64.58-73.46%, 55.24-67.37%.

Embodiment 3

[0081] Embodiment 3: when banana tissue-cultivated seedlings are transplanted, No. 1, No. 2 and No. 3 composite microbial inoculants are mixed into the tissue-cultured seedling transplanting substrate or soil respectively, and each cubic seedling-raising substrate Mix in 0.5kg or 2.5kg or 5kg of compound microbial inoculum, and then spray compound microbial inoculum No. 1, compound microbial inoculum No. 2 and compound microbial inoculum No. 3 200 times liquid or 500 times liquid or 1000 times liquid, a total of 3 times, the untreated banana seedlings were transplanted to the same field as the blank control and the banana seedlings of the treatment group, and managed according to conventional methods. 30 days, 60 days, 90 days, and 120 days after transplanting, the disease of banana wilt disease was investigated respectively. The survey results showed that the incidence of banana wilt disease was higher than that of the control group 30 days, 60 days, 90 days, and 120 days afte...

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Abstract

The invention relates to a biological control method and a biocontrol agent for banana vasicular wilt, pertaining to the field of biological control over banana vasicular wilt. The method comprises following steps: colonizing antagonistic bacteria of vasicular wilt during transplant of banana tissue culture seedlings, colonizing antagonistic bacteria of vasicular wilt during transplant of banana seedling land, and colonizing antagonistic bacteria of vasicular wilt during growth of banana plants. The biocontrol agent is an antagonistic compound microorganism bacterium agent for banana vasicular wilt and antagonistic bio-organic fertilizer for banana vasicular wilt. The compound microorganism bacterium agent is prepared by compound of two or multiple kinds from five agents including azotobacter chroococcum YKT41, burkholderia vietnamiensis P418, bacillus megaterium Ap25, trichoderma viride LTR-2 and trichoderma harzianum T110W. The bio-organic fertilizer is prepared by 5-25% of the compound microorganism bacterium agent, 10-30% of protein residues, 5-300% of lignite, 10-25% of biochemical fulvic acid, 10-35 % of biogical charcoal and added with 0.5-2.5% of calcium carbonate according to the above mixture. The biological control method and the biocontrol agent for banana vasicular wilt have following advantages: the biological control method and the biocontrol agent helps effectively prevent and control infection and morbidity of bacteria for banana vasicular wilt and are eco-friendly; and the method is easy and convenient.

Description

Technical field: [0001] The invention belongs to the field of biological control of banana diseases, and in particular relates to a biological control method of banana wilt and a biological control preparation thereof. Background technique: [0002] Banana Fusarium wilt is a disease that infects the vascular bundles of banana plants. Its main features are the wilting of the diseased plants and the discoloration and rot of the vascular bundles. It is a devastating disease and is an international plant quarantine object. Type (Fusariumoxysporumf.sp.cubense) infection. Fusarium oxysporum (F.oxysporum) is a worldwide distribution of soil-borne pathogenic fungi with a wide range of hosts, which can cause more than 100 plant wilts in melons, Solanaceae, bananas, cotton, legumes and flowers. . Fusarium oxysporum belongs to Imperfectifungi, from the order Moniliales, Tuberculariaceae, and Fusarium. Pathogenic bacteria survive in the soil for several years, generally reducing yiel...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G13/00A01G17/00A01N63/04A01P3/00C12N1/14C12N1/20C12R1/125C12R1/01C12R1/885C12R1/11
CPCA01G13/00A01G17/005A01N63/30C12N1/14C12N1/20Y02A40/22Y02P60/21
Inventor 张廷金闫春丽尹忠仁李文荣王建光陈穗云扈进冬李纪顺杨合同李秀军
Owner 昆明保腾生化技术有限公司
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