Method for tissue culture and rapid propagation of euphorbia pekinensis

A tissue culture and rapid technology, applied in the field of plant reproduction, can solve the problems of resource destruction, harsh growth environment requirements, wild spurge resources on the verge of depletion, etc., achieving the effect of robust seedlings, high survival rate, and solving the problem of large-scale seedling breeding.

Inactive Publication Date: 2016-06-15
GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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Problems solved by technology

In recent years, its resources have been severely damaged, and the requirements for the growth environment are harsh. With the continuous increase of market demand, wild euphorbia resources are on the verge of exhaustion, and it is difficult to realize large-scale cultivation in production.

Method used

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  • Method for tissue culture and rapid propagation of euphorbia pekinensis
  • Method for tissue culture and rapid propagation of euphorbia pekinensis
  • Method for tissue culture and rapid propagation of euphorbia pekinensis

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Embodiment Construction

[0011] The technical solutions of the present invention will be further described below in conjunction with the examples.

[0012] A rapid propagation method of Euphorbia tissue culture, comprising the following steps:

[0013] (1) Selection and disinfection of explants: take Euphorbia euphorbia terminal buds as explants, soak them in 2% detergent solution for 5-10 minutes, rinse them with linear tap water for 5-15 minutes, and disinfect the surface in 75% ethanol for 30-30 minutes. 45s, 0.1%Hgcl 2 (Add 2~3 drops of Tween-20) Disinfect for 8~10min, rinse with sterile water for 3~5 times, and finally absorb the surface water with sterile filter paper to obtain explants, in which sterile water is autoclaved distilled water.

[0014] (2) Terminal bud differentiation to obtain clustered bud rapid propagation culture: Inoculate the explants obtained in step (1) into MS medium at a culture temperature of 23-26°C, light intensity of 1400-2000lux, and light time of 10 After culturi...

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Abstract

The invention discloses a method for tissue culture and rapid propagation of euphorbia pekinensis. The method comprises the following steps: (1) taking terminal buds of the euphorbia pekinensis as explants to be disinfected; (2) placing the disinfected explants in an MS minimal medium for induction to obtain multiple shoots; (3) placing the multiple shoots in an MS strong seedling medium for strong seedling cultivation to obtain strong plants; (4) placing the strong plants in an MS rooting medium for cultivation to obtain complete rooted seedlings. By adopting the method provided by the invention, the obtained seedlings are strong and high in survival rate, a large number of high-quality seedlings of the euphorbia pekinensis can be provided in a short period, and the problem of large-scale seedling cultivation of the euphorbia pekinensis is effectively solved.

Description

technical field [0001] The invention relates to a plant propagation method, in particular to a rapid propagation method of Euphorbia tissue culture. Background technique [0002] Euphorbia, Euphorbiaceae plant Euphorbia ( Euphorbia pekinensis Rupr.), a perennial evergreen herb. Root cylindrical, 20-30 cm long. The flowering period is from May to August, and the fruiting period is from June to September. Widely distributed in the whole country (except Taiwan, Yunnan, Tibet and Xinjiang), especially in the north. Growing on hillsides, thickets, roadsides, wastelands, grasses, forest margins and sparse forests. The root is used as medicine, which has high medicinal value, about 100 yuan / kg. It has the functions of dispelling water and laxative, reducing swelling and dissipating stagnation, treating edema mainly, and has the effect of dredging menstruation. It is used for the treatment of chronic nephritis, ascites or Other diseases such as liver cirrhosis and ascites. In ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 李翠张占江吕惠珍缪剑华韦莹韦坤华李林轩王一诺肖冬
Owner GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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