Application of a microbial strain for repairing heavy metal polluted soil

A technology for contaminated soil and cadmium-contaminated soil, which is applied in the field of microbial strains for repairing heavy metal-contaminated soil, can solve the problems of soil damage, high implementation cost, pollution, etc., and achieve the effect of small soil disturbance, high implementation cost and good effect

Active Publication Date: 2019-11-05
CHINA NAT TOBACCO QUALITY SUPERVISION & TEST CENT +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0008] The present invention has certain limitations for existing physical and chemical methods for treating heavy metal-contaminated soil, high implementation cost, serious damage to soil, and other pollution problems

Method used

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  • Application of a microbial strain for repairing heavy metal polluted soil
  • Application of a microbial strain for repairing heavy metal polluted soil
  • Application of a microbial strain for repairing heavy metal polluted soil

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1: the screening of microbial strain of the present invention

[0029]Liquid-phase enrichment method: Weigh an appropriate amount of cadmium-contaminated soil sample and add it to a conical flask filled with LB liquid medium, and add an appropriate amount of glass beads. Shake the triangular flask at 28-30° C., 150-170 rpm; transfer the soil mixture into a centrifuge tube, and take the supernatant after centrifugation as the source of cadmium-inactivated microorganisms. The above supernatant was inoculated into cadmium-containing LB liquid medium, and cultured with shaking at 28-30° C. and 150-170 rpm. Draw 1-2mL with a sterile pipette and transfer to another enrichment culture Erlenmeyer flask. After three transfers in this way, streak the plate on LB solid medium and inorganic salt solid medium respectively to isolate single colonies.

[0030] The supernatants were diluted 10 -1 、10 -2 、10 -3 、10 -4 、10 -5 、10 -6 、10 -7 、10 -8 , absorb an appropri...

Embodiment 2

[0031] Embodiment 2: the identification of microbial strain of the present invention

[0032] The DNA of the strain was extracted according to the steps of the TIANamp bacteria DNA kit (TIANamp bacteria DNA kit) for species identification. Among the results with the highest matching degree, the measured 16S rDNA sequence of the strain has 100% homology with the 16S rDNA sequence of Burkholderia cepacia, which can determine the strain For Burkholderia cepacia (Burkholderia cepacia).

Embodiment 3

[0033] Embodiment 3: the cadmium passivation characteristic of bacterial strain of the present invention

[0034] The isolated pure culture strains were inoculated in LB liquid medium, and cultured with shaking at 28-30°C and 160-170rpm for 18-20h, in which a group of bacterial cells were first inactivated at 121°C for 15 minutes, and then 6000 -8000rpm low-temperature centrifugation for 15-20min to collect dead bacteria as a dead cell passivation agent; another group of bacterial cells was directly centrifuged at 3000-5000rpm low-temperature for 20-30min to collect live bacteria and washed 2-3 times with sterile water , as a passivating agent for living cells. The two groups of bacteria were added to the cadmium-containing (50-300mg / L Cd 2+ ) in an aqueous solution for adsorption experiments, the results are as follows figure 1 shown. according to figure 1 It can be seen from the results that both of them have strong ability to remove cadmium ions, but the adsorption capa...

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Abstract

The invention relates to the field of microorganisms, and discloses a microorganism strain for repairing heavy metal polluted soil and an application thereof. The deposit number of the microorganism strain of the present invention is CGMCC No.10843. The invention selects a Burkholderia cepacia strain from soil polluted by heavy metal cadmium, which can passivate exchangeable cadmium in the soil and significantly reduce its content, and has extremely strong tolerance to cadmium, It has little disturbance to soil, has strong bioremediation function, and can improve the activity of soil dehydrogenase.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to a microbial strain for repairing heavy metal polluted soil and its application. Background technique [0002] Due to long-term mining, metal smelting and application of phosphorus-containing pesticides, a large amount of heavy metal pollutants are enriched in the soil. Heavy metal polluted soil is concealed, cumulative and hysteresis, so it is not easy to attract attention. In recent years, the problem of soil pollution by heavy metals in my country has become increasingly prominent, posing a great threat to the soil quality of cultivated land and the health and safety of people's livelihood. According to the survey results of the national soil pollution situation by the Ministry of Environmental Protection and the Ministry of Land and Resources of China, the soil in my country is seriously polluted by heavy metals such as cadmium, arsenic, lead, chromium, and nickel. / 5, among whi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20B09C1/10C12R1/01
CPCB09C1/10C12N1/205C12R2001/01
Inventor 何声宝张威罗安娜刘楠王英元冯晓民
Owner CHINA NAT TOBACCO QUALITY SUPERVISION & TEST CENT
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