A construction method of hplc characteristic map of traditional Chinese medicine for treating wind-heat and cold
A characteristic map and anemopyretic cold technology, applied in the field of medicine, can solve the problem of lack of detection of multiple main chemical components, cumbersome methods, inability to detect and identify a single treatment for anemopyretic cold, and inability to monitor the quality of raw materials, intermediates, and finished products And other issues
Active Publication Date: 2017-10-31
吉林修正药业新药开发有限公司
View PDF8 Cites 0 Cited by
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
[0004] 1. In the methods specified in the Pharmacopoeia, there is no comprehensive and systematic detection of the main chemical components of the multi-flavored medicinal materials
[0005] 2. The current non-quality inspection method cannot monitor the quality of raw materials, intermediates, and finished products
[0006] In summary, in the prior art, a method for treating wind-heat-cold preparation (patent No. CN 104623082 A) has defects such as cumbersome methods, inability to quickly and comprehensively detect and identify a kind of wind-heat-cold preparation
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View moreImage
Smart Image Click on the blue labels to locate them in the text.
Smart ImageViewing Examples
Examples
Experimental program
Comparison scheme
Effect test
Embodiment 1
[0032] Example 1: Establishment of a characteristic map method for treating Fengreganmao preparation.
[0033] 1 Instrument: UltiMate 3000DGLC high performance liquid chromatograph, ultraviolet DAD detector; Mettler AE240 1 / 100,000 analytical balance (Shanghai Mettler-Toledo Instrument Co., Ltd.); AB204-E electronic balance (Shanghai Mettler-Toledo Instrument Co., Ltd.); Lido Instrument Co., Ltd.), etc.
[0034] 2 Reagents and reagents
[0035] 2.1 Reagents: methanol, etc. are analytically pure, phosphoric acid and acetonitrile are chromatographically pure, and water is ultrapure water.
[0036] 2.2 Reagent: chlorogenic acid, forsythiaside B, liquiritin, luteolin, forsythin, baicalin, glycyrrhizic acid (all from China Food and Drug Appraisal Research Institute), a preparation for treating wind-heat and cold. Batch number: 120601, 120602, 120603, 130501, 130502, 130503, 130504, 130505, 141201, 141202, 141203 (from Jilin Xiuxiu Pharmaceutical New Drug Development Co., Ltd.). ...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More PUM
Login to View More Abstract
The invention relates to a construction method of an HPLC specific chromatogram of traditional Chinese medicines treating wind-heat cold. The construction method includes the steps of: preparing a test sample solution, preparing a reference substance solution, specifying chromatographic conditions, precisely sucking 10 [mu]l of the test sample solution and 10 [mu]l of a medicine solution respectively, injecting the solutions into a liquid chromatographic instrument, and performing HPLC test to obtain the HPLC specific chromatogram. Under the same chromatographic conditions, the chemical components in various traditional Chinese medicines can be detected to determine quality consistency of a lot of batches according to the similarity of finger-print spectrum, and furthermore, the method can be used for searching problems in process operations fundamentally. The similarities of the finger-print spectrum and a reference spectrum are both higher than 0.9. The method effectively shows the quality between the batches of a finish product and can be used for comprehensively monitoring product quality, thereby avoiding simplicity and one-sidedness during quality control. The method is convenient, quick, stable and precise and has good repeatability.
Description
technical field [0001] The invention belongs to the field of medicine, and in particular relates to a quality control method of a preparation for treating anemopyretic cold. Background technique [0002] The characteristic map of traditional Chinese medicine can comprehensively and comprehensively reflect the relative relationship of the components contained in it, and better reflect the complexity and correlation of the components of traditional Chinese medicine. It has the characteristics of obvious characteristics, strong specificity, and good reproducibility. Application, as a quality control method of Chinese herbal medicines and their extracts, characteristic maps have become a technique widely accepted at home and abroad to evaluate the quality of Chinese medicines. [0003] Previously, there was no clear detection method for a preparation for treating wind-heat and cold (patent number CN 104623082 A), and the Chinese Pharmacopoeia was mostly used for the medicinal fl...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More Application Information
Patent Timeline
Login to View More Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02
CPCG01N30/02G01N2030/027
Inventor 高嵩白冰郭文英吴萍李桂峰
Owner 吉林修正药业新药开发有限公司