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Method for analyzing amentoflavone in selaginella tamariscina

A technology of double flavonoids and an analysis method, which is applied in the field of laboratory testing, can solve problems such as non-compliance with customs rapid testing, and achieve the effect of improving the speed of analysis

Inactive Publication Date: 2016-06-22
WUZHOU INST FOR FOOD & DRUG CONTROL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The operation time of the pharmacopoeia extraction and analysis method is often more than 6 hours, which does not meet the requirements of rapid inspection by customs and quality inspection departments

Method used

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  • Method for analyzing amentoflavone in selaginella tamariscina
  • Method for analyzing amentoflavone in selaginella tamariscina

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Embodiment 1

[0029] Crush Selaginella through a pulverizer, pass through a No. 3 sieve, weigh about 0.2g, accurately weigh it, mix it with 1g of diatomaceous earth, and put it into the ASE5ml extraction tank with a filter membrane in advance, then add an appropriate amount of diatomaceous earth , shake gently to make it at the same level as the pool mouth, and tighten the upper cover of the extraction pool. After the extraction, transfer the extract to a 25ml volumetric flask, dilute to the mark with methanol, centrifuge at 15000r / min for 3min, take the supernatant, and enter it into HPLC for determination.

[0030] The extraction conditions (see table 1) are:

[0031] Table 1

[0032]

[0033]

[0034] Analysis method is LC liquid chromatography, liquid chromatography analysis conditions:

[0035] a) Instrument: Thermo dual-ternary liquid chromatograph (U-3000)

[0036] b) Chromatographic column: ThermoSyncronis C183*100mm3um, that is, a carbon-18 chromatographic column with a di...

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Abstract

The invention discloses a method for analyzing amentoflavone in selaginella tamariscina. The method is characterized by comprising the following steps: 1, pulverizing the selaginella tamariscina, and then obtaining extract liquor through an ASE extraction method, wherein equipment adopted according to the ASE extraction method is an ASE rapid extraction instrument, the extraction condition is that extracting solvent is methyl alcohol, the extraction temperature is 110 DEG C, static extraction time is 5 minutes, the flushing volume is 80%, and the number of static circulation times is 3; 2, conducting dilution and centrifugation on the extract liquor obtained in step 1, so that supernatant is obtained; 3, testing the amentoflavone content in the supernatant obtained in step 2 through a liquid chromatography. The method for analyzing the amentoflavone in the selaginella tamariscina is high in detection speed and high in detection accuracy.

Description

technical field [0001] The invention relates to the field of laboratory testing, in particular to an analysis method for biflavonoids from Selaginella spicosa. Background technique [0002] In 2010, the Pharmacopoeia recorded the method for extracting the biflavones from Selaginella spica: Take about 0.2g of the powder of this product (passed through a No. 3 sieve), accurately weigh it, put it in a stoppered conical flask, add 50ml of methanol precisely, and weigh it. Heat to reflux for 5 hours, let cool, weigh again, make up the lost weight with methanol, shake well, filter, and take the filtrate to obtain the final product. The chromatographic conditions are as follows: octadecylsilane bonded silica gel is used as filler; methanol is used as mobile phase A, 0.1% phosphoric acid solution is used as mobile phase B, and gradient elution is carried out according to different mobile phase ratios; the detection wavelength is 330nm. The number of theoretical plates should not be...

Claims

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Application Information

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IPC IPC(8): G01N30/88
CPCG01N30/88G01N2030/027G01N2030/8809
Inventor 陈学松欧妮陈翠玲钟水桥李仁乔
Owner WUZHOU INST FOR FOOD & DRUG CONTROL
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