Purple sweet potato virus-free seedling water culture fast breeding method

A technology of virus-free seedlings and purple sweet potatoes, which is applied in the field of crop seedlings, can solve the problems of high production cost of original seeds, high production environment requirements, and harsh test conditions, and achieve the effects of low cost, simple process, and short production cycle

Inactive Publication Date: 2016-06-29
NINGXIA ACADEMY OF AGRI & FORESTRY SCI
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Problems solved by technology

[0002] At present, there are two main methods for the propagation of virus-free purple potato seedlings. One is to propagate the branches of the original virus-free potato by cuttings. This method takes a long time to propagate and has a low survival rate. The production cost is high; the other is that the virus-free test-tube seedlings of purple sweet potato are propagated in MS-based medium. This method has a faster propagation speed and a higher survival rate, but the production environment requires high requirements, harsh test conditions, and the cost of the medium High, which in turn leads to high production costs of the original species

Method used

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specific Embodiment approach

[0019] Step 1: Sterilize, cut off the stem tips of virus-free purple potato seedlings with a length of 2.0cm to 3.0cm, fix them in the holes of the foam board with sponges or cotton balls, float in 1‰carbendazim solution, soak the roots for 30min, Rinse with water 3~5 times.

[0020] Step 2: rooting culture, the row spacing of cuttings is 5cm×6cm, put it into the water culture seedling tray and let it stand and float in the rooting nutrient solution. The depth of the rooting nutrient solution is 5cm-6cm. Cultivate for 5-7 days under the conditions of 7200lx, light 12h / d, and humidity 70%-75%, and complete rooting. The formula of rooting nutrient solution is: (NH 4 ) 2 SO 4 0.0~20㎎ / L; KNO 3 0.0~2.5㎎ / L; K 2 SO 4 0.0~2.5㎎ / L; MgSO 4 0.0~15.0㎎ / L; KH 2 PO 4 0.0~3.0㎎ / L; Ca(NO 3 ) 2 0.0~2.0㎎ / L; FeSO 4 0.0~1.0㎎ / L; Na 2 -EDTA0.0~1.0㎎ / L; H 3 BO 3 0.0~3.0㎎ / L; MnCl 2 0.0~0.5㎎ / L; ZnSO 4 0.0~0.03㎎ / L; CuSO 4 0.0~0.02㎎ / L; Na 2 MoO 4 0.0~0.02㎎ / L.

[0021] The rooting nutrien...

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Abstract

A method for hydroponic rapid propagation of virus-free purple potato seedlings, belonging to the field of crop seedlings, the steps are: 1. Disinfection: cut the stem tip of virus-free purple potato seedlings to a length of 2.0cm to 3.0cm, and fix it on a foam board with a sponge or cotton ball In the hole, float in 1‰ carbendazim solution, soak the roots for 30 minutes, and rinse with water for 3 to 5 times; 2. Rooting: Put the sterilized seedlings in step 1 into the water culture seedling tray and let them float Cultivate in the rooting nutrient solution for 5-7 days, and the aquatic roots grow to 1.0 cm to 3.0 cm; 3. Cultivation: the purple potato seedlings with water roots in step 2 are cultivated in the strong seedling nutrient solution for 12-15 days to grow into 4 to 5 leaves are used as mother seedlings; 4, cut 2.0cm to 3.0cm long stem tips on the mother seedlings cultivated in step 3, and carry out disinfection, rooting and cultivation; 5, cut 8 to 10 stem tips from the mother seedlings in step 3 Transplanted as seedlings after the second time. The virus-free purple potato seedlings propagated by the method of the invention have low production cost and good quality.

Description

technical field [0001] The invention belongs to the technical field of crop seedling cultivation, in particular to a hydroponic rapid propagation method for virus-free purple potato seedlings. Background technique [0002] At present, there are two main methods for the propagation of virus-free purple potato seedlings. One is to propagate the branches of the original virus-free potato by cuttings. This method takes a long time to propagate and has a low survival rate. The production cost is high; the other is that the virus-free test-tube seedlings of purple sweet potato are propagated in MS-based medium. This method has a faster propagation speed and a higher survival rate, but the production environment requires high requirements, harsh test conditions, and the cost of the medium High, which in turn leads to high production costs of the original species. [0003] On July 22, 2015, the Chinese Patent Office disclosed the publication number 104782487A of "A Method for Rapid...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G31/00
CPCA01G31/00
Inventor 朱金霞张源沛郑国保孔德杰李苗曲继松张丽娟
Owner NINGXIA ACADEMY OF AGRI & FORESTRY SCI
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