Composition obtained by fermenting folium eriobotryae with probiotics and preparing method and application of composition
A technology of loquat leaves and probiotics, applied in the directions of bacteria, application, lactobacillus and the like used in food preparation, can solve the problems of simple and extensive, minimal effective ingredients, waste, etc., and achieves reduction of production costs, long shelf life, and improved utilization. Effect
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Embodiment 1
[0031] Example 1: The weight g / volume ml percentage of the loquat leaf powder in the distilled water soaking solution is 6-10%.
[0032] A kind of preparation of the composition of probiotic fermented loquat leaf, concrete method is as follows:
[0033] Take dried loquat leaves, crush through a 100-mesh sieve, take loquat leaf powder and soak in distilled water for 30 minutes according to the weight g / volume ml ratio of 6%;
[0034] Add glucose 0.7% (weight g / volume ml), peptone 0.4% (weight g / volume ml), yeast extract 0.13% (weight g / volume ml), yeast powder 0.07% (weight g / volume ml) to the soaking solution , inorganic salt (K 2 HPO 4 ·3H 2 O0.1g / 100ml, KH 2 PO 4 0.1g / 100ml, FeSO 4 ·7H 2 O5.0mg / 100ml, ZnSO 4 ·7H 2 O4.4mg / 100ml, MgSO 4 ·7H 2 O50mg / 100ml), sucrose 3.0% (weight g / volume ml) and sucralose 0.008% (weight g / volume ml). Adjust the pH to 6.5-7.0, and sterilize at about 115°C for 50 minutes to obtain sterile loquat leaf extract;
[0035] When the temper...
Embodiment 2
[0038] A kind of preparation of the composition of probiotic fermented loquat leaf, concrete method is as follows:
[0039] Take dried loquat leaves, crush through a 100-mesh sieve, take loquat leaf powder and soak in distilled water for 30 minutes according to the weight g / volume ml ratio of 8%;
[0040] Add glucose 0.6% (weight g / volume ml), peptone 0.3% (weight g / volume ml), yeast extract 0.1% (weight g / volume ml), yeast powder 0.05% (weight g / volume ml) to the soaking solution , inorganic salt (K 2 HPO 4 ·3H 2 O0.1g / 100ml, KH 2 PO 4 0.1g / 100ml, FeSO 4 ·7H 2 O5.0mg / 100ml, ZnSO 4 ·7H 2 O4.4mg / 100ml, MgSO 4 ·7H 2 O50mg / 100ml), sucrose 3.0% (weight g / volume ml) and sucralose 0.008% (weight g / volume ml). Adjust the pH to 6.5-7.0, and sterilize at about 115°C for 50 minutes to obtain sterile loquat leaf extract;
[0041] When the temperature of the extract drops to about 39°C, insert the pre-cultivated Bifidobacterium infantis (AS1.853), Bifidobacterium adolescent (A...
Embodiment 3
[0044] A kind of preparation of the composition of probiotic fermented loquat leaf, concrete method is as follows:
[0045] Take dried loquat leaves, crush through a 100-mesh sieve, take loquat leaf powder and soak in distilled water for 30 minutes according to the weight g / volume ml ratio of 10%;
[0046] Add glucose 0.5% (weight g / volume ml), peptone 0.2% (weight g / volume ml), yeast extract 0.08% (weight g / volume ml), yeast powder 0.03% (weight g / volume ml) to the soaking solution , inorganic salt (K 2 HPO 4 ·3H 2 O0.1g / 100ml, KH 2 PO 4 0.1g / 100ml, FeSO 4 ·7H 2 O5.0mg / 100ml, ZnSO 4 ·7H 2 O4.4mg / 100ml, MgSO 4 ·7H 2 O50mg / 100ml), sucrose 3.0% (weight g / volume ml) and sucralose 0.008% (weight g / volume ml). Adjust the pH to 6.5-7.0, and sterilize at about 115°C for 50 minutes to obtain sterile loquat leaf extract;
[0047] When the temperature of the extract drops to about 39°C, insert the pre-cultured Bifidobacterium bifidum (isolated from normal human body, identif...
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