Separation and extraction method for cytomembrane microvesicles (MVs) and exosomes (EXs)
An extraction method and technology for microvesicles, which are applied in the field of medical invention and can solve problems such as research and development limitations
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[0017] The following is a specific implementation case of this project. The reagents and materials used in the subordinate applications can be obtained through market purchase channels. The pH of PBS used in the present invention is 7.2.
[0018] Such as Figure 4 As shown, extract 3ml of peripheral blood and put it in a 2ml sterile tube containing 3.3% sodium citrate; add 6ml of filtered PBS; mix well and perform centrifugation. Separation conditions: speed 400r / s, 35min, 4 ℃; take the uppermost liquid as plasma; take 1ml of plasma for centrifugation, separation conditions: speed 2000r / s, 20min, 4℃, used to remove platelets; take supernatant for centrifugation, separation conditions: speed 20000r / s, 120min, 4°C, the obtained precipitate was suspended in 700μl filtered PBS for NTA analysis of the total number of cMVs; the remaining supernatant was centrifuged, separation conditions: 169000r / s, 6h, 4°C, the obtained precipitate was used 700 μl of filtered PBS was suspended f...
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