Genetic identification method of cotton germplasm based on ssr marker and capillary electrophoresis
A capillary electrophoresis, genetic identification technology, applied in the application of molecular marker-assisted selection breeding, based on SSR markers and capillary electrophoresis high-throughput genetic identification field, can solve problems such as difficult analysis methods, achieve high resolution and wide application range , the analysis results are accurate and reliable
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[0037] Refer to attached figure 1 , figure 2 , image 3 , 95 kinds of cotton varieties were selected as experimental materials. A method for genetic identification of cotton germplasm based on SSR markers and capillary electrophoresis, characterized in that it comprises the following steps:
[0038] 1. Cotton genome DNA extraction, for the experimental material of above-mentioned 95 cotton variety data, carry out the extraction of DNA respectively:
[0039] (1) Take 1 young cotton leaf and put it into a 1.5 ml centrifuge tube, add 0.6 ml extraction buffer to make 500 mL formula as follows: 0.35M Glucose: 34.68g, 0.1M Tris.HCl: 50 mL, 5mM Na. EDTA: 5 mL, 2% PVP: 100 mL, 1% β-Me: 5 mL, dd Water: 340 mL, grind with electric drill;
[0040] (2) Centrifuge at 10,000 rpm (Eppendorf) for 15 min (4°C), discard the supernatant; add 0.6 ml 65°C preheated lysis buffer to the pellet (500 mL formula is as follows: 1.4M NaCl: 40.908g, 0.1M Tris .HCl: 50mL, 20mMNa, EDTA: 20mL, 2%CTAB: ...
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