Extraction device and applications thereof to extraction, oil extraction and measurement for oil content of microbial cell

Abstract

The invention belongs to an experimental instrument and an application and especially relates to an extraction device and applications thereof to extraction, oil extraction and measurement for oil content of microbial cell. The extraction device comprises a hopper body, a guide pipe, a faucet cavity and a faucet, wherein the upper end of the hopper body is opening; the guide pipe is arranged on the lower part of the hopper body; the faucet cavity is arranged in the middle of the guide pipe; the upper and lower ends of the guide pipe are communicated with the faucet cavity; the faucet is in rotating sealing fit with the inner surface of the faucet cavity and is provided with a through hole; the guide pipe and the hopper body above the faucet cavity are transparent. The extraction device further comprises a centrifuge bottle with a bottle opening at the top end, wherein the centrifuge bottle is made of an elastic material with a self-resetting function; an interface in sealing communication with the bottle opening at the upper end of the centrifuge bottle is formed on the outer side of the lower part of the guide pipe. The experimental instrument is used for solving the problems of complex applied equipment and complex operation of the extraction and measuring method in the prior art and has the advantages of simple equipment structure, high-speed operation, small measuring error, and the like.

Description

technical field [0001] The invention belongs to experimental equipment and its application, in particular to an extraction device and its application in extraction, oil extraction and determination of microbial cell oil content. Background technique [0002] In recent years, single-cell lipids have received extensive attention as an emerging biological resource, with applications ranging from feed, food supplements, pharmaceuticals, and energy. In the process of vigorously developing microbial resources, it has been found that many types of microorganisms can synthesize a large amount of oil, which has the characteristics of high oil content. The development and utilization of microbial oil has gradually become a research hotspot in the world today. Microbial oils have a wide range of uses. For example, some microbial oils contain a large amount of medium- and long-chain saturated fatty acid esters, which can be processed to obtain biodiesel; while some microbial oils contai...

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Problems solved by technology

Although the oil extraction efficiency of the improved assay method has been improved to a certain extent, the following defects still exist: the one is that the repeated transfer and centrifugation of freezing, lyophilization, manual grinding, soaking and sample solution and extraction liquid in the above steps make the operation very difficult. It is cumbersome, and the measurement time is very long. It takes about 1 day to measure the total lipid content of a sample, which is time-consuming and laborious; second, the methanol / chloroform organic solvent used in many methods is toxic and harmful to human health; In the process of processing, some methods require special equipment, such as high-pressure homogeneous wall-breaking method requires high-pressure homogenizer, ultrasonic-assisted wall breaking requires ultrasonic equipment, algae need freeze-drying machine for freeze-drying, etc., and most of these instruments are large-scale The equipment is relatively expensive; fourth, the broken cell residues in the oil extraction process of some methods are easy to redisperse after centrifugation, making it difficult to separate the solid-liquid two-phase; fifth, some methods need to separate the liquid-liquid two-phase The extraction phase is repeatedly sucked and transferred with a straw. The operation is very cumbersome, and it is easy to spill during the transfer. The sample loss is serious, resulting in inaccurate measurement results. Sixth, some extraction mixtures are emulsified, and the separation of the two phases is very difficult. Centrifugation assists stratification, and then uses a separatory funnel to separate the two phases, so repeated liquid transfer between the centrifuge tube and the separatory funnel causes sample loss, and the operation is cumbersome, time-consuming and laborious

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