Methods and compositions to produce rice resistant to ACCase inhibitors

A rice, resistance technology, applied in the field of producing ACC enzyme inhibitor-resistant rice and compositions, which can solve the problems of expensive, time-consuming, etc.

Inactive Publication Date: 2016-07-06
RICETEC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Obtaining herbicide resistance genes and introducing them into the rice genome with otherwise favorable traits and different resistances is challenging, unpredictable, time-consuming and expensive, but essential to meeting the world's growing food needs. necessary

Method used

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  • Methods and compositions to produce rice resistant to ACCase inhibitors
  • Methods and compositions to produce rice resistant to ACCase inhibitors
  • Methods and compositions to produce rice resistant to ACCase inhibitors

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] Example 1: Results of ML0831265-01493 Response to Quizalofop Herbicide Rates

[0062] The response of rice lines with the G2096S mutation to quizalofop herbicide was tested by testing a series of herbicide dosages. level of resistance. Treatment with different herbicide doses such as Figure 6 shown. All treatments were applied at the 2-3 leaf stage. Plots were evaluated 21 days after application. The spray was applied at a rate of 10 gal / acre containing 1% vegetable oil. Each treatment was assessed as percent injury compared to unsprayed control plots.

[0063] The source of the G2096S mutation is the ML0831265-01493 strain. A seed sample from ML0831265-01493 was deposited with ATCC. This line is resistant to some ACCase herbicides due to a mutation in the ACCase gene that results in an amino acid change at position 2096 of serine instead of glycine, and is similar to R0146 except for this resistance.

[0064] Four different selections were made on ML0831265-0...

Embodiment 2

[0065] Example 2: Results of Response of Rice Lines with G2096S Mutation to ACC Inhibitor Herbicide Doses

[0066] The response of a rice line (ML0831265-01493) with the G2096S mutation to different ACCase-inhibiting herbicides was examined. Choose a group of herbicides ( Figure 7 ) and applied it to three rice lines with the G2096S mutation and the non-mutated original variety R0146. The response of R0146 and mutant lines as Figure 7 shown. Herbicides were applied at twice the labeled level, except for quizalofop and clethodim, which were applied at twice the amount suitable to kill rice.

[0067] All treatments were applied at the 2-3 leaf stage, approximately 20 days after seed planting. Plots were evaluated 21 days after application. The spray was applied at a rate of 10 gal / acre containing 1% vegetable oil. Each treatment was assessed as percent injury compared to unsprayed control plots.

[0068] A seed sample from ML0831265-01493 was deposited with ATCC. This ...

Embodiment 3

[0071] Example 3: The mutant rice line ML0831265-01493 has no obvious difference in characteristics from the non-mutant R0146 rice, the difference is only that the former is ACCase inhibitor-resistant rice

[0072] In the study plot, the mutant rice line ML0831265-01493 was observed side by side with the original non-mutant line R0146. No observable differences were identified. Plants show the same growth pattern. Plant health and robustness also appeared similar without any detectable difference. Some of the measured characteristics also revealed new significant differences between the mutant line ML0831265-01493 and the non-mutated line R0146 (Table 2). The G2096S mutation in line ML0831265-01493 showed no negative effects on normal plant growth or fitness.

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Abstract

Mutant rice resistant/tolerant to ACCase inhibiting herbicides, in particular FOP herbicides, are listed in Table 1. The ACCase inhibitng herbicides used for selection include quizalofop. An exemplary mutant rice tolerant to an ACCase herbicide is disclosed, with a rice genome having G2096S or the equivalent, in the carboxyl transferase domain of the ACCase coding gene, using the Black-Grass numbering system. This mutation shows differential response to FOPs vs. DIMs herbicides, and a greater differential with comparable non-resistant rice lines. Methods to control weeds and methods to produce herbicide resistant rice including transgenic rice, are disclosed.

Description

Background technique [0001] This application claims priority to US Provisional Application No. 61 / 510,585, filed July 22, 2011, and US Provisional Application No. 61 / 541,832, filed September 30, 2011, both of which are incorporated herein by reference. The present application describes and discloses novel rice species characterized by tolerance / resistance to ACCase inhibitor herbicides and exhibiting other characteristics beneficial to rice crops. Also disclosed are methods of controlling weeds using herbicide-resistant rice in the field and methods of producing herbicide-resistant rice using, for example, a transgene encoding a mutated ACCase. [0002] sequence listing [0003] This application contains a Sequence Listing, which has been submitted in ASCII format via EFS-Web, and is hereby incorporated by reference in its entirety. The ASCII file was created on July 20, 2012, named 115760_SEQ_ST25.txt, and has a size of 32,768 bytes. [0004] The value of the rice crop ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/04A01C1/00A01H5/00A01G22/22A01H5/10
CPCC12Y604/01002C12N9/93A01N37/46A01H5/10C12N15/8274A01H6/4636C12N15/8241
Inventor M·欣加S·格里芬M·S·穆恩R·D·拉斯马森F·奎瓦斯
Owner RICETEC
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