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A kind of method that utilizes aquatic protein and feather meal to ferment and prepare small growth-promoting peptides for poultry

A technology for hydrolyzing feather meal and promoting growth, applied in the application, animal feed, animal husbandry and other directions, can solve the problems of high production cost and large amount, and achieve the effects of low production cost, increased energy consumption, and reduced production cost

Active Publication Date: 2019-08-13
GUANGDONG OCEAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the enzymatic hydrolysis method requires a large amount of protease, and the production cost is high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Embodiment 1: the heat-resistant facultative anaerobic bacteria capable of producing keratinase preserved on a slant Bacillus methylotrophicus HL-4 was inserted into the LB liquid medium cooled after sterilization, and cultured at 55°C and 150r / min for 12h, and prepared into Bacillus methylotrophicus The HL-4 seed solution is reserved. Fresh tilapia scraps are minced for later use; 20 kg of minced tilapia scraps, 5 kg of hydrolyzed feather meal and 100 kg of water are added to the enzymatic hydrolysis tank, and 50 g of them are added to the enzymatic hydrolysis tank Glucose, 520 grams of calcium chloride, 320 grams of zinc chloride, 500 grams of ferrous chloride and 550 grams of dipotassium hydrogen phosphate are mixed to obtain a fermentation medium; add 8 kg to the fermentation medium in the enzymolysis tank Bacillus methylotrophicus HL-4 seed liquid; control the fermentation temperature at 55°C to 60°C, the rotation speed at 50rpm to 100rpm, and the time for...

Embodiment 2

[0028] Embodiment 2: the heat-resistant facultative anaerobic bacteria capable of producing keratinase preserved on slant Bacillus methylotrophicus HL-4 was inserted into the LB liquid medium cooled after sterilization, and cultured at 55°C and 150r / min for 12h, and prepared into Bacillus methylotrophicus The HL-4 seed solution is reserved. Mince fresh Antarctic krill for later use. Add 25 kg of minced Antarctic krill, 6 kg of hydrolyzed feather meal and 100 kg of water into the enzymolysis tank, and add 60 g of glucose, 450 g of calcium chloride, 380 g of zinc chloride, Mix 555 grams of ferrous chloride and 650 grams of dipotassium hydrogen phosphate to obtain a fermentation medium; add 8 kilograms to the fermentation medium in the enzymolysis tank Bacillus methylotrophicusHL-4 seed liquid; control the fermentation temperature at 55°C to 60°C, the rotation speed at 50rpm to 100rpm, and the time for 66h; after the fermentation, the fermentation liquid is filtered to rem...

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PUM

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Abstract

The invention discloses a method of utilizing fermentation of aquatic product protein and feather meal to prepare growth-promoting small peptide for poultry.The method mainly includes following steps: mincing fresh aquatic product leftovers; adding the minced aquatic product leftovers and hydrolyzed feather meal into an enzymolysis tank, adding nutrient and water, and mixing well to form a fermentation medium; inoculating Bacillus methylotrophicus HL-4 capable of generating keratinase into the enzymolysis tank, and performing enzymolysis and fermentation at the same time; after fermentation is finished, filtering fermentation liquid to remove precipitate to obtain supernate, and subjecting the supernate to concentration and spray drying to obtain the growth-promoting small peptide for poultry.The small peptide prepared by the method is rich in aquatic product protein and keratinase peptide, can remarkably promote growing of poultry and has good application prospect.

Description

technical field [0001] The invention relates to a method for preparing poultry growth-promoting small peptides by fermenting aquatic protein and feather meal, and belongs to the field of aquatic product processing and utilization. Background technique [0002] Research results at home and abroad show that adding a certain amount of small peptides to the diet can improve the body's utilization of protein and amino acids in the diet, and improve the growth performance of animals. The growth-promoting effect of small peptides in poultry has received extensive attention. The research results of "Effects of Adding Small Plant Peptides in Diets on Growth, Slaughter Performance and Serum Biochemical Indexes of Yellow Feather Broilers" (Feed Industry, 2009, No. 12) showed that adding plant small peptides can improve the growth performance of yellow feather chickens. The research results of "The Effects of Adding Small Peptide Products in the Diet on the Production Performance and N...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23K10/26A23K10/12A23K10/30
Inventor 洪鹏志刘唤明周春霞杨萍
Owner GUANGDONG OCEAN UNIVERSITY