Cage for mouse in waking state and application of cage

An awake state and cage technology, applied in applications, medical science, sensors, etc., can solve problems such as expensive anesthetics, reduced food intake of mice, and lower body temperature, achieving strong practicability, operability, and fast detection speed , cheap effect

Inactive Publication Date: 2016-07-13
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, mice need to be anesthetized or sedated during magnetic resonance imaging to keep the mice absolutely still. Anesthesia or sedation will cause side effects such as reduced food intake and lower body temperature in mice, and there is a risk of death
In addition, anesthesia is time-consuming and expensive

Method used

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  • Cage for mouse in waking state and application of cage
  • Cage for mouse in waking state and application of cage
  • Cage for mouse in waking state and application of cage

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Embodiment 1: the making of mouse cage

[0020] The typical size of the design of the mouse cage: the outer diameter of the cylinder 1 is 29mm, the inner diameter is 23mm, the diameter of the side wall hole is 5mm, and the total length is 200mm; the length of the plug 2 is 40mm, the outer diameter is 29mm, and the inner diameter is 23mm; The outer diameter is 22mm, the groove width is 4mm ( figure 2 ). Made of polytetrafluoroethylene according to the size of the drawing.

Embodiment 2

[0021] Example 2: Carrying out nuclear magnetic resonance analysis using a mouse cage

[0022] Five mice were purchased with a mass of 21.54 ± 5.65 g. Put the mouse into the barrel 1, and fix the mouse by screwing in the plug 2 and advancing the push-pull rod 3. Using the MiniMR-Rat magnetic resonance imaging analyzer, using the CPMG sequence, the transverse relaxation curve of the mouse was measured, and the parameters were set as: 90-degree pulse width P 1 :13μs, 180 degree pulse width P 2 :26μs, resampling waiting time Tw:3000ms, analog gain RG1:15, digital gain DRG1:3, preamplifier gain PRG:1, resampling times NS:8, number of echoes NECH:5000, receiver bandwidth SW : 200KHz, the control parameter RFD of the start sampling time: 0.01ms, the time delay DL1: 0.1ms, and obtain the transverse relaxation curve of each sample ( image 3 ). Use the IR sequence to measure the longitudinal relaxation curve of the mouse, and the parameters are set as: 90-degree pulse width P 1 :...

Embodiment 3

[0023] Example 3: Magnetic resonance imaging using a mouse cage

[0024] Select a mouse weighing 17.93g, put the mouse into cylinder 1, and fix the mouse by screwing in plug 2 and advancing push-pull rod 3. Using MiniMR-Rat magnetic resonance imaging analyzer, using SPINECHO (SE) sequence for magnetic resonance imaging, the parameters are set as: slices: 4, FovRead: 100mm, FovPhase: 100mm, slicewidth: 2.5mm, slicegap: 0.5mm, Readsize: 256 , Phasesize: 192, Average: 4, TR: 500ms, TE: 20ms, the magnetic resonance imaging ( Figure 5 ). Depend on Figure 5 The tissues and organs of the mice can be clearly seen, indicating that the cage can be used for magnetic resonance imaging of mice.

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PUM

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Abstract

The invention relates to a cage for a mouse in the waking state. The cage comprises a barrel, a plug and a push-pull rod, is made from polytetrafluoroethylene and is cylindrical as a whole, two ends of the barrel are open, a round groove with an internal thread is formed in one end surface of the barrel, the plug with an external thread is in threaded connection in the position, where the round groove with the internal thread is located, of the barrel, a ventilating hole is formed in the center of the plug, an inverted circular-truncated-cone-shaped groove is formed in the middle of the plug, an opening is formed in the side wall surface of the other end of the barrel, one end of the push-pull rod extends into the other end of the barrel, the outer wall surface of the push-pull rod is closely attached to the inner wall surface of the barrel, a screw hole is formed in the side wall surface of the push-pull rod, a screw is in threaded connection with the screw hole along the opening of the side wall surface of the barrel, a groove is formed in the push-pull rod, and 12 round holes are formed in the side wall surface of the barrel. The cage is simple in structure, firm, durable and capable of being used for fixing the mouse in the waking state for nuclear magnetic resonance detection, anesthetization is not required, repeated detection can be performed, and the cage has the higher application value.

Description

technical field [0001] The invention belongs to the technical field of experimental animal appliances, and in particular relates to a cage for mice in an awake state and an application thereof. Background technique [0002] Mice are the most commonly used experimental animals. In the study of specific molecular imaging of diseases such as tumors, it is necessary to observe the experimental effect by magnetic resonance imaging (Document 1: Daishun Ling etal. .Soc.2014:136,5647-5655). However, at present, mice need to be anesthetized or sedated during magnetic resonance imaging to keep the mice absolutely still. Anesthesia or sedation will cause side effects such as reduced food intake and lower body temperature in mice, and there is a risk of death . In addition, anesthesia is time-consuming and anesthetics are expensive. [0003] The present invention fully recognizes the importance and necessity of magnetic resonance detection in mice in an awake state, and recognizes th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61B5/055
Inventor 谭明乾谢威扬耿少特马小军
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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