A kind of culture medium and method for producing conidia by solid fermentation of Mortierella chinensis

Abstract

The invention relates to a culture medium and a method suitable for producing conidia through solid fermentation of hiresutella sinensis X.J.Liu Y.L. Guo. Peat soil is taken as a major substrate an a proper amount of a basic nutrient solution is added, so that the solid-state fermentation medium which is suitable for the growth of the hiresutella sinensis is prepared; the hiresutella sinensis can achieve growth in the medium, and the conidia are easy to separate and extract, so that the large-scale production of the conidia through the solid fermentation of the hiresutella sinensis X.J.Liu Y.L. Guo is achieved; in addition, the culture medium can facilitate infection of bat moth larvae and is applicable to the breeding of cordyceps sinensis; meanwhile, the invention also provides a method for producing the conidia through the solid fermentation of the hiresutella sinensis X.J.Liu Y.L. Guo which is cultivated in the peat soil medium; and by appropriately changing external condition and stimulating the asexual reproduction of the hiresutella sinensis X.J.Liu Y.L. Guo, the amount of spores (the conidia) is increased, and / or by adding a conidium inducing agent, the asexual reproduction of the hiresutella sinensis X.J.Liu Y.L. Guo is induced, so that the amount of the spores is increased.

Description

technical field [0001] The invention relates to a culture medium and method suitable for producing conidia by solid fermentation of Mortierella chinensis. technical background [0002] It has been confirmed that the anamorph of Cordyceps sinensis is Hirsutella sinensis X.J.Liu, Y.L.Guo, Y.X.Yu&W.Zeng (Jiang Yi and Yao Yijian 2003; Li Zengzhi et al. 2000; Liu Xijing et al. 1989). China's Trichospores liquid fermentation has realized industrial production, and developed products such as Bailing Capsules and Bailing Tablets (Dong et al.2015; Dong Caihong et al. 2015). Time cultivation leads to an increase in the probability of contamination, and at the same time increases the complexity and risk of metabolites (Li Shulin et al. 2014). Therefore, simulating the plateau ecological breeding of Cordyceps sinensis has become an urgent need. [0003] The breeding of Cordyceps sinensis first needs to obtain a large number of conidia for infecting bat moth larvae. Due to the complex...

AI Technical Summary

Problems solved by technology

Due to the complex characteristics of Mortierella chinensis species, under the existing fermentation process, there are only a few reports on the use of Mortierella chinensis to prepare conidia. The methods in the literature are all obtained by solid slant culture Conidia (Li Yuling 2002; Wang Wei et al. 2002; Wang Can 2012; Li Lingling et al. 2014; Zhu Zhigang 2014)

Existing method for preparing conidia from Mortierella sinensis is basically carried out on solid slant medium in test tubes, petri dishes or jars, the medium has poor permeability, and it is difficult for Mortierella sinensis to grow inside the culture medium, and the culture medium In actual production, it cannot be applied to the breeding of Cordyceps sinensis. Limited by the material of the medium itself and the space of the culture container, it is difficult to separate the medium from the Mortierella chinensis colony. The separation and extraction of conidia requires the colony to be stripped from the medium, and the mycelia Separation from conidia is cumbersome and difficult to scale up

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