Micro-channel structure and micro-fluid chip for achieving two-dimensional fluid power accumulation by using single-way sheath liquid

A technology of fluid dynamics and micro-channels, which is applied in the direction of laboratory containers, laboratory utensils, chemical instruments and methods, etc., which can solve the problems of reducing detection accuracy and stability, small cross-section, and inability to realize high-speed detection of biological particles, etc. problem, to achieve the effect of ensuring the two-dimensional focusing effect and maintaining the stability of the liquid flow

Active Publication Date: 2016-08-17
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, these designs cannot achieve high-speed detection of biological particles, and at the same time reduce the accuracy and stability of detection. In addition, some micro-channel structures can achieve two-dimensional focusing, and have the characteristics of high flow rate and small cross-section

Method used

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  • Micro-channel structure and micro-fluid chip for achieving two-dimensional fluid power accumulation by using single-way sheath liquid
  • Micro-channel structure and micro-fluid chip for achieving two-dimensional fluid power accumulation by using single-way sheath liquid
  • Micro-channel structure and micro-fluid chip for achieving two-dimensional fluid power accumulation by using single-way sheath liquid

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0082] The first layer of micro-channels and the third layer of micro-channels communicate with the second layer of micro-channels and have the same outer profile to ensure that the pressure of the sheath fluid flow b therein is the same as that of the liquid in the second-layer micro-channels. The flow pressure changes synchronously and maintains the stability of the liquid flow in the system; the two arcs in the first layer of microchannels (the upper longitudinal focused flow channel 120) or the third layer of microchannels (the lower longitudinal focused flow channel 130) The flow channel profile at the intersection of the micro-channel (the left arc channel part 122 (132) and the right arc channel part 123 (133)) needs to maintain an appropriate length L in the front and rear directions, and the length will increase if the length is too long. The sheath liquid flow b in the first layer of microchannels and the third layer of microchannels squeezes the sample flow a lateral...

Embodiment 2

[0089] In this embodiment, the heights of the first layer of microchannels and the third layer of microchannels are equal to meet the requirement of focusing the sample flow a at the vertical center. The height of the second layer of microchannels can be different from the heights of the first layer and the third layer of microchannels, keeping the height of the second layer of microchannels unchanged, by increasing or decreasing the first layer of microchannels and the second layer of microchannels at the same time The height of the three-layer microchannel can increase or decrease the flow rate of the sheath fluid b in the two-layer microchannel, thereby enhancing or weakening the longitudinal focusing effect on the sample flow a (correspondingly weakening and then strengthening the lateral focus on the sample flow a focus effect).

[0090] Specifically, the height of the second layer of microchannels in this embodiment is 150 μm, the heights of the first layer and the third...

Embodiment 3

[0096] Such as Figure 7 As shown, in this embodiment, the flow rate of sample flow a is 1 μL / s, and the flow rate of sheath fluid b is 120 μL / s. After focusing, the flow velocity of sample flow a reaches 5.1 m / s, and sample flow a is located in the center of the flow channel. The front end surface of the straight channel portion 121 ( 131 ) is a micro-channel structure 100 with a flat, angled or arc-shaped surface, and other dimensions are the same as those of the micro-channel structure 100 in Embodiment 1.

[0097] When the front end of the linear channel part 121 (131) adopts a flat surface, the sheath liquid flow b in the first layer of microchannels and the third layer of microchannels has a limited longitudinal focusing effect on the sample flow a, and the focused sample flow a The cross-sectional shape is approximately a rectangle of 6 μm×50 μm (width×height).

[0098] When the front end surface of the straight channel part 121 (131) adopts an angled surface, the shea...

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Abstract

The invention discloses a micro-channel structure and a micro-fluid chip with the micro-channel structure for achieving two-dimensional fluid power accumulation by using a single-way sheath liquid. The micro-channel structure comprises a sample flow channel, an upper longitudinal accumulated flow channel, a lower longitudinal accumulated flow channel and two transverse accumulated flow channels, wherein the upper longitudinal accumulated flow channel is arranged above the sample flow channel in an overlapped manner; the lower longitudinal accumulated flow channel is arranged below the sample flow channel in the overlapped manner; the two transverse accumulated flow channels are respectively arranged on the left side and right side of the sample flow channel; the width of each transverse accumulated flow channel is gradually reduced from back to front in a liquid flowing direction; and the maximum width of each transverse accumulated flow channel is greater than that of the sample flow channel. According to the micro-channel structure for achieving two-dimensional fluid power accumulation by using the single-way sheath liquid, disclosed by the embodiment of the invention, two-dimensional accumulation of a sample flow can be achieved, the sample flow can be prevented from being dispersed by the sheath liquid, the liquid flow can be kept stable, and the two-dimensional accumulation effect of the sample flow can be ensured.

Description

technical field [0001] The invention relates to the technical field of microfluidic channels, and more specifically, to a microfluidic channel structure and a microfluidic chip that utilizes a single channel of sheath fluid to realize two-dimensional hydrodynamic focusing. Background technique [0002] Combined with flow cytometry, microfluidic chips are often used to detect biological particles such as cells, embryos, RNA, DNA, protein particles, microorganisms, and viruses. In the detection, the microfluidic chip needs to stably focus the sample flow containing biological particles at the center of the microchannel to improve the accuracy, sensitivity and stability of the detection. Restricted by processing technology, microchannels can only realize complex curved structures in the plane, but in the vertical direction, they are mostly vertical tube walls, or inclined or bowl-shaped tube walls determined by the processing technology. Therefore, the micro-channel structure ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01L3/00
CPCB01L3/00B01L3/5027
Inventor 尤政赵精晶李滨
Owner TSINGHUA UNIV
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