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Culture medium and culture method for inducing morphological transformation of Paecilomyces hepiali strain SH-1, and strain

A technology based on Paecilomyces bat moth and SH-1, which is applied in the direction of microorganism-based methods, biochemical equipment and methods, fungi, etc., can solve the problems of difficult selection and low recognition degree, and achieve high recognition degree and practical High performance and easy to use

Inactive Publication Date: 2016-08-24
QINGHAI ACAD OF ANIMAL SCI & VETERINARY MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, if multiple strains are studied or cultivated in the same space environment at the same time, after artificial cultivation, because the color of Paecilomyces hematalis strain SH-1 is similar to that of most other strains, its recognition degree is low. When it is necessary to use When , it is very difficult to select

Method used

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  • Culture medium and culture method for inducing morphological transformation of Paecilomyces hepiali strain SH-1, and strain
  • Culture medium and culture method for inducing morphological transformation of Paecilomyces hepiali strain SH-1, and strain
  • Culture medium and culture method for inducing morphological transformation of Paecilomyces hepiali strain SH-1, and strain

Examples

Experimental program
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Embodiment 1

[0061] The culture medium for inducing the morphological transformation of Paecilomyces hematalis strain SH-1 provided by the present embodiment is mainly made of the following raw materials, and every 100ml of liquid culture medium includes,

[0062]

[0063] This embodiment also provides a method for inducing morphological transformation of the Paecilomyces hematalis strain SH-1, comprising culturing the Paecilomyces hematalis strain SH-1 on a medium.

[0064] This embodiment also provides the bacterial strain obtained by cultivating the above-mentioned medium using the above-mentioned method.

Embodiment 2

[0066] The culture medium for inducing the morphological transformation of Paecilomyces hematalis strain SH-1 provided by the present embodiment is mainly made of the following raw materials, and every 100ml of liquid culture medium includes,

[0067]

[0068] This embodiment also provides a method for inducing the morphological transformation of the Paecilomyces hematalis strain SH-1, comprising culturing the Paecilomyces hematalis strain SH-1 on a medium, wherein the ambient temperature is 20°C.

[0069] This embodiment also provides the bacterial strain obtained by cultivating the above-mentioned medium using the above-mentioned method.

Embodiment 3

[0071] This example provides a method for inducing the morphological transformation of Paecilomyces hematalis strain SH-1, comprising culturing the Paecilomyces hematalis strain SH-1 on a medium.

[0072] Among them, the culture conditions are: the amount of liquid in a 500mL glass culture bottle is 200mL (medium), the inoculum size is 10%, the flask is shaken on a shaker at 160r / min for 10min, and the temperature is placed in a culture room at 20°C for cultivation After 60 days, the pH was 5.8.

[0073] The formula for calculating the transition probability is:

[0074]

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Abstract

The invention provides a culture medium and culture method for inducing morphological transformation of a Paecilomyces hepiali strain SH-1, and a strain, relating to the technical field of strain culture. Every 100ml of the liquid culture medium is mainly prepared from 2-5g of carbon source, 20-27g of nitrogen source (comprising eggs), 0.05-0.31g of inorganic salt (selected from one or more of magnesium sulfate and dipotassium hydrogen phosphate), and the balance of water. The culture medium is simple in components, and can be used for culturing the recognizable Paecilomyces hepiali strain with the same species as the Paecilomyces hepiali strain SH-1. The method provided by the invention is convenient to operate, has low requirements for environmental conditions, and is easy to implement. The strain provided by the invention is pink, has high recognition degree, and is easy to select.

Description

technical field [0001] The invention relates to the technical field of bacterial strain cultivation, in particular to a culture medium for inducing morphological transformation of Paecilomyces hematalis strain SH-1, a cultivation method and a bacterial strain. Background technique [0002] Cordyceps sinensis is a complex of insects and fungi formed by Cordyceps sinensis parasitizing on the larvae of insects of the genus Batamothus. Cough due to deficiency and dyspnea, labored cough and hemoptysis, impotence and nocturnal emission, soreness of waist and knees". Cordyceps sinensis is sweet and warm in taste, mild in nature, warm but not dry, nourishing but not stagnant. It has more extensive medicinal value than other kinds of tonic Chinese medicines, and has no side effects. [0003] Cordyceps sinensis is also called Paecilomyces bat moth, and its artificially cultivated mycelium composition and content are very similar to natural Cordyceps sinensis, which is the most suitab...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12R1/79
CPCC12N1/14C12N1/145C12R2001/79
Inventor 张宗豪李玉玲刘欣徐海峰
Owner QINGHAI ACAD OF ANIMAL SCI & VETERINARY MEDICINE
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