Somatic embryo rapid propagation and seedling growing method for camellia chekiangoleosa

A technology of safflower camellia and rapid breeding seedlings, which is applied in the field of plant tissue culture, can solve the problems that Zhejiang safflower camellia tissue culture technology has not been reported, and the natural reproduction rate of camellia oleifera is low, and achieve good practical effect

Active Publication Date: 2016-08-31
浙江萧建集团有限公司
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  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The natural reproduction rate of camellia oleifera is low, so tissue culture rapid propagation technology can be used to solve the

Method used

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  • Somatic embryo rapid propagation and seedling growing method for camellia chekiangoleosa
  • Somatic embryo rapid propagation and seedling growing method for camellia chekiangoleosa
  • Somatic embryo rapid propagation and seedling growing method for camellia chekiangoleosa

Examples

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Example Embodiment

[0027] Example 1

[0028] A method for rapid propagation of seedlings from somatic embryos of Zhejiang safflower oil tea, the steps are as follows:

[0029] 1) Explant pretreatment

[0030] The seeds harvested in October 2014 from Zhejiang Safflower Camellia in Wuyi Mountain, Fujian Province were selected as materials, and the seed shells were carefully removed to avoid damage to the seeds. Soak the seeds in water for 1.5h. After that, the seed coat is removed. Wash with detergent and rinse with running water for 2h.

[0031] 2) Sterilization of safflower oil tea fruit and establishment of aseptic system

[0032] Wipe the ultra-clean workbench with 75% alcohol and turn on the UV lamp to sterilize it for 20 minutes. Put the seeds rinsed with running water into a sterile bottle with tweezers, pour 75% alcohol, so that the seeds are completely immersed in the alcohol, stir and sterilize for 30 seconds, pour out the alcohol and rinse once with sterile water. Pour in 0.1% mercury and st...

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Abstract

The invention discloses a somatic embryo rapid propagation and seedling growing method for camellia chekiangoleosa. The method comprises the steps of 1, pretreatment of explants; 2, sterilization of camellia chekiangoleosa fruits and establishment of a sterile system; 3, inoculation and induction of calluses; 4, induction of somatic embryos; 5, induction of secondary embryos; 6, differentiation of the secondary embryos into strong seedlings. According to the somatic embryo rapid propagation and seedling growing method for camellia chekiangoleosa, N6+NAA 1.0 mg/L+6-BA 1.0 mg/L+2,4-D 1.0 mg/L is used as a medium for inducing the embryonic calluses; after inoculation, cotyledon pieces on the medium begin to expand after about 7 d of culture, and a large quantity of faint yellow and dense embryonic calluses with smooth and lustrous surfaces begin to appear around the cotyledon pieces after about 15 d; after the embryonic calluses are transferred to a somatic embryo induction medium, the somatic embryos begin to appear after about 20 d; through proliferation and differentiation culture of the secondary embryos, sprouts and roots appear through differentiation after about 15 d, and finally the strong seedlings are formed. An efficient camellia chekiangoleosa somatic embryogenesis system is established through the method, and a large quantity of seedlings is provided for camellia chekiangoleosa.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a method for rapidly growing somatic embryo seedlings of Zhejiang safflower camellia oleifera. Background technique [0002] Zhejiang safflower camellia, alias: Zhejiang red camellia. scientific name: Camellia chekiangoleosa . Camellia, the genus Camellia, is a unique tree species in my country. Distributed in southern Zhejiang, eastern Jiangxi, and high mountains on the borders of Zhejiang, Fujian, and Anhui provinces. Zhejiang safflower camellia has two uses: ornamental and oil. The plant has beautiful shape, emerald green leaves, beautiful and huge safflowers, bright colors, fruit shaped like apples, full of red fruits in summer and autumn, strong cold resistance, and is an excellent parent for camellia resistance breeding. This tree species has a high yield of tea seed oil and good oil quality, and the quality and yield of camellia oil are better...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 续晨温强徐雯蔡小宁贲爱玲杨平徐立军赵俊丽段淑蓉华春
Owner 浙江萧建集团有限公司
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