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Application of fish pentraxin

A technology of penetrase and penetrase, applied in the field of molecular biology, can solve the problem of little research on the function of fish PTX1

Inactive Publication Date: 2016-08-31
INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the function of PTX1 in fish has been poorly studied

Method used

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  • Application of fish pentraxin

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Preparation of Recombinant Protein of Penetrin PTX1

[0016] 1) Construction of plasmid pEtPTX1 expressing penetrase PTX1 recombinant protein:

[0017] The sequence of penetrantin PTX1 in the present invention has been reported (GenBank accession number XM_008323050.1). Penetrancerin PTX1 has a typical penetrancein domain (amino acids 19-214). The construction of pEtPTX1 was as follows: the cDNA of tongue sole was used as template, and PCR amplification was carried out with primers F1 and R1. The PCR conditions were: pre-denatured template DNA at 94°C for 60s, then 94°C for 40s, 55°C for 60s, 72°C for 60s, 5 cycles, then changed to 94°C for 40s, 61°C for 60s, 72°C for 60s, 30 cycles. The PCR product was purified with the Tiangen DNA Product Purification Kit and ligated with the PCR cloning carrier TBSC-T (purchased from "Tiangen Biochemical Technology Co., Ltd.", Beijing) at room temperature for 2-4 hours, and the ligation mixture was transformed into Escherichia coli...

Embodiment 2

[0024] Bacteriostatic Application of Recombinant Penetratein

[0025] Step 1) Bacterial suspension preparation

[0026] Grow Pseudomonas fluorescens in LB medium to OD 600 0.8, then centrifuged (5000g, 4°C, 10min), collected the bacteria, suspended in TBSC to a final concentration of 10 4 cfu / ml is the suspension of Pseudomonas fluorescens.

[0027] The Pseudomonas fluorescens is preserved in the General Microbiology Center CGMCC of the China Microbiological Culture Collection Management Committee, the preservation number is: CGMCC NO.2329, the preservation date is 2008.1.9, and the classification is named as Pseudomonas fluorescens (Pseudomonas fluorescens), address : Institute of Microbiology, Chinese Academy of Sciences, Datun Road, Chaoyang District, Beijing.

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Abstract

The invention relates to the field of molecular biology, particularly relates to application of a fish pentraxin and discloses the application of the fish pentraxin to preparation of antimicrobial agents. The pentraxin is a pentraxin recombinant protein; the pentraxin recombinant protein is a recombinant protein prepared by the expression of escherichia coli. When the recombinant pentraxin is applied to the preparation of the antimicrobial agents, the growth of the bacteria can be significantly inhibited.

Description

technical field [0001] The invention relates to the field of molecular biology, in particular to the application of a fish penetrant. Background technique [0002] Pentraxin PTX1 (Pentraxin 1) belongs to the pentraxins family and is an extracellular secreted protein with a molecular weight of about 24.7 kDa, which consists of a signal peptide region and a PTX domain region (pentraxin domain). As a typical body fluid pattern recognition molecule, on the one hand, PTX1 can bind a variety of microorganisms, such as fungi, yeast, bacteria, and parasites, by recognizing polysaccharides and phosphorylcholine on the cell membrane; on the other hand, PTX1 can bind a variety of Nuclear substances, such as DNA, histones, chromatin, and small ribonucleic acid proteins. Binding or agglutination of PTX1 with ligands can activate the classical complement pathway, trigger inflammatory responses, and enhance opsonization. At the same time, the PTX1-ligand complex can bind to FcγRI type I,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/17A61P31/04C12N15/12C12N15/70C07K14/46
CPCA61K38/1709C07K14/461
Inventor 孙黎王婷
Owner INST OF OCEANOLOGY - CHINESE ACAD OF SCI