Natural active ingredient extracted and separated from leaves and stems of panax quinuefolium L and application of natural active ingredient
An American ginseng and its isolated technology are applied to natural active ingredients and their application fields, which can solve problems such as waste of resources, achieve the effects of improving body immunity, enhancing the body's ability to resist fatigue and hypoxia, and highlighting substantive characteristics.
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Embodiment 1
[0027] Embodiment 1: the preparation of American ginseng stem and leaf extract
[0028] The extract of this example is prepared through the following steps:
[0029] Step S1, harvesting fresh American ginseng stems and leaves, and drying in the shade;
[0030] Step S2, drying the American ginseng stems and leaves that have been harvested and dried in the shade at 50°C, crushing them through a 20-mesh sieve to obtain fine powder of stems and leaves;
[0031] Step S3, extracting the stem and leaf fine powder with 80% ethanol under reflux for 3 times, each time for 2 hours, and collecting the filtrate by filtration;
[0032] Step S4, the filtrate is concentrated under reduced pressure until it has no alcohol smell, purified and enriched with AB-8 macroporous adsorption resin, first eluted with 15% ethanol for 8 column volumes, and then with 75% ethanol for 14 column volumes, Collect 75% ethanol eluate;
[0033] In step S5, the 75% ethanol eluate is concentrated under reduced p...
Embodiment 2
[0034] Embodiment 2: the preparation of American ginseng stem and leaf extract
[0035] The extract of this example is prepared through the following steps:
[0036] Step S1, harvesting fresh American ginseng stems and leaves, and drying in the shade;
[0037] Step S2, drying the American ginseng stems and leaves that have been harvested and dried in the shade at 50°C, crushing them through a 20-mesh sieve to obtain fine powder of stems and leaves;
[0038] Step S3, extracting the stem and leaf fine powder with 80% ethanol under reflux for 3 times, each time for 2 hours, and collecting the filtrate by filtration;
[0039] Step S4, the filtrate is concentrated under reduced pressure until it has no alcohol smell, purified and enriched with AB-8 macroporous adsorption resin, first eluted with 10% ethanol for 8 column volumes, and then with 75% ethanol for 14 column volumes, Collect 75% ethanol eluate;
[0040] In step S5, the 75% ethanol eluate is concentrated under reduced p...
Embodiment 3
[0041] Embodiment 3: the preparation of American ginseng stem and leaf extract
[0042] The extract of this example is prepared through the following steps:
[0043] Step S1, harvesting fresh American ginseng stems and leaves, and drying in the shade;
[0044] Step S2, drying the American ginseng stems and leaves that have been harvested and dried in the shade at 50°C, crushing them through a 20-mesh sieve to obtain fine powder of stems and leaves;
[0045] Step S3, extracting the stem and leaf fine powder with 80% ethanol under reflux for 3 times, each time for 2 hours, and collecting the filtrate by filtration;
[0046] Step S4, the filtrate is concentrated under reduced pressure until it has no alcohol smell, purified and enriched with AB-8 macroporous adsorption resin, first eluted with 20% ethanol for 8 column volumes, and then with 75% ethanol for 14 column volumes, Collect 75% ethanol eluate;
[0047] In step S5, the 75% ethanol eluate is concentrated under reduced p...
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