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Natural active ingredient extracted and separated from leaves and stems of panax quinuefolium L and application of natural active ingredient

An American ginseng and its isolated technology are applied to natural active ingredients and their application fields, which can solve problems such as waste of resources, achieve the effects of improving body immunity, enhancing the body's ability to resist fatigue and hypoxia, and highlighting substantive characteristics.

Inactive Publication Date: 2016-09-07
WEIHAI PINHE BIOLOGICAL SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This is a huge waste of resources

Method used

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  • Natural active ingredient extracted and separated from leaves and stems of panax quinuefolium L and application of natural active ingredient
  • Natural active ingredient extracted and separated from leaves and stems of panax quinuefolium L and application of natural active ingredient
  • Natural active ingredient extracted and separated from leaves and stems of panax quinuefolium L and application of natural active ingredient

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Embodiment 1: the preparation of American ginseng stem and leaf extract

[0028] The extract of this example is prepared through the following steps:

[0029] Step S1, harvesting fresh American ginseng stems and leaves, and drying in the shade;

[0030] Step S2, drying the American ginseng stems and leaves that have been harvested and dried in the shade at 50°C, crushing them through a 20-mesh sieve to obtain fine powder of stems and leaves;

[0031] Step S3, extracting the stem and leaf fine powder with 80% ethanol under reflux for 3 times, each time for 2 hours, and collecting the filtrate by filtration;

[0032] Step S4, the filtrate is concentrated under reduced pressure until it has no alcohol smell, purified and enriched with AB-8 macroporous adsorption resin, first eluted with 15% ethanol for 8 column volumes, and then with 75% ethanol for 14 column volumes, Collect 75% ethanol eluate;

[0033] In step S5, the 75% ethanol eluate is concentrated under reduced p...

Embodiment 2

[0034] Embodiment 2: the preparation of American ginseng stem and leaf extract

[0035] The extract of this example is prepared through the following steps:

[0036] Step S1, harvesting fresh American ginseng stems and leaves, and drying in the shade;

[0037] Step S2, drying the American ginseng stems and leaves that have been harvested and dried in the shade at 50°C, crushing them through a 20-mesh sieve to obtain fine powder of stems and leaves;

[0038] Step S3, extracting the stem and leaf fine powder with 80% ethanol under reflux for 3 times, each time for 2 hours, and collecting the filtrate by filtration;

[0039] Step S4, the filtrate is concentrated under reduced pressure until it has no alcohol smell, purified and enriched with AB-8 macroporous adsorption resin, first eluted with 10% ethanol for 8 column volumes, and then with 75% ethanol for 14 column volumes, Collect 75% ethanol eluate;

[0040] In step S5, the 75% ethanol eluate is concentrated under reduced p...

Embodiment 3

[0041] Embodiment 3: the preparation of American ginseng stem and leaf extract

[0042] The extract of this example is prepared through the following steps:

[0043] Step S1, harvesting fresh American ginseng stems and leaves, and drying in the shade;

[0044] Step S2, drying the American ginseng stems and leaves that have been harvested and dried in the shade at 50°C, crushing them through a 20-mesh sieve to obtain fine powder of stems and leaves;

[0045] Step S3, extracting the stem and leaf fine powder with 80% ethanol under reflux for 3 times, each time for 2 hours, and collecting the filtrate by filtration;

[0046] Step S4, the filtrate is concentrated under reduced pressure until it has no alcohol smell, purified and enriched with AB-8 macroporous adsorption resin, first eluted with 20% ethanol for 8 column volumes, and then with 75% ethanol for 14 column volumes, Collect 75% ethanol eluate;

[0047] In step S5, the 75% ethanol eluate is concentrated under reduced p...

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PUM

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Abstract

The invention discloses a natural active ingredient extracted and separated from leaves and stems of panax quinuefolium L and an application of the natural active ingredient. The natural active ingredient is prepared by the following steps: S1, harvesting fresh leaves and stems of panax quinuefolium L, and drying the leaves and stems of panax quinuefolium L in the shade; S2, drying the leaves and stems of panax quinuefolium L which are dried in the shade by virtue of an oven, crushing the leaves and stems of panax quinuefolium L and filtering the crushed leaves and stems of panax quinuefolium L by virtue of a sieve of 20-30 meshes so as to obtain fine powder of the leaves and stems; S3, conducting heat reflux extraction o the fine powder of the leaves and stems by virtue of 70-90% of ethanol for 2-3 times, wherein each time lasts for 1-2h, and collecting filtrates; S4, decompressing and concentrating the filtrates until no alcohol smell exists, enriching by virtue of AB-8 macroporous resin, eluting by 6-10 column volumes by virtue of 10-20% of ethanol firstly, and then eluting by 12-16 column volumes by virtue of 70-80% of ethanol, collecting an eluent of eluting by the70-80% of ethanol; and S5, decompressing and concentrating the eluent of eluting by the70-80% of ethanol, and spray-drying, so that an extract of the leaves and stems of panax quinuefolium L is obtained. The extract disclosed by the invention is relatively good in activity, and the extract is suitable for preparing medicines, health care foods or health care products; and the extract disclosed by the invention, which is prepared from waste leaves and stems of panax quinuefolium L, achieves full utilization of plant processing waste and improves an industrial value; therefore, the invention has substantive distinguishing features and remarkable progress.

Description

technical field [0001] The invention belongs to the field of plant extracts, and relates to the extraction, separation and application of natural active ingredients, in particular to the natural active ingredients extracted and separated from American ginseng stems and leaves as raw materials and their application. Background technique [0002] The stems and leaves of American ginseng are recognized as precious medicinal materials, which have medicinal value that its roots do not have, but they have not been effectively developed in the past due to the limitations of extraction equipment and methods. [0003] Taking the American ginseng planting industry in Wendeng City, Shandong Province as an example, in the process of planting American ginseng, Yaonong only studied how to increase the yield of American ginseng, but neglected the development and utilization of American ginseng stems and leaves. According to measurements, the content of active ingredients such as saponins a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/258A61P37/04A61P39/00A61P3/10A61P9/12A61P3/06A61P35/00A23L33/105
CPCA61K36/258A23V2002/00A61K2236/333A61K2236/39A61K2236/55A23V2200/324A23V2200/31A23V2200/328A23V2200/326A23V2200/3262A23V2200/308A23V2250/21A23V2300/14
Inventor 姜军亭
Owner WEIHAI PINHE BIOLOGICAL SCI & TECH
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