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Method for determination of platelet aggregation rate

A technology of platelet aggregation and determination method, which is applied in the field of determination of platelet aggregation rate, can solve the problems of complicated and time-consuming specimen preparation, large amount of whole blood samples, etc., reduce the risk of operators contacting samples, overcome complex preparation and operational errors Reduced effect

Inactive Publication Date: 2016-09-14
山东泰利信医疗科技有限公司
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AI Technical Summary

Problems solved by technology

Using a single platelet-rich plasma to achieve photoelectric turbidimetry (LTA for short) to measure platelet aggregation rate can overcome two major defects in conventional detection methods: complicated and time-consuming specimen preparation and a large amount of whole blood samples

Method used

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  • Method for determination of platelet aggregation rate
  • Method for determination of platelet aggregation rate
  • Method for determination of platelet aggregation rate

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Embodiment 1

[0021] In the assay method of the platelet aggregation rate of the present invention, the platelet aggregation rate is measured according to photoelectric turbidimetry, and the measuring steps are:

[0022] A, collect the patient's blood sample provided by the blood collection department (the blood collection department of the medical unit) (according to the detection standard dosage collection, usually 2.5mL-3mL; the blood sample in the prior art is usually 5mL), the blood sample is anticoagulated , generate anticoagulant whole blood, and centrifuge the anticoagulant whole blood to prepare platelet-rich plasma; The ratio of blood is 1:9; inject the anticoagulated whole blood into the sample tube and then transfer it to the centrifuge for centrifugation, and centrifuge at low speed (500-800 rpm, centrifuge for 5 minutes) to separate and prepare PRP (that is, platelet-rich plasma) , in the present embodiment, also can adopt the index of control centrifugal force to realize sepa...

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Abstract

The invention relates to a method for determination of platelet aggregation rate. The method conducts determination on the basis of photoelectric turbidimetry, and comprises the determination steps of: placing prepared platelet-rich plasma onto a sample plate device, imbibing the platelet-rich plasma, injecting into a test cup of a test movement device, and reading the transmittance data of the platelet-rich plasma sample; imbibing a platelet aggregation inducer, injecting into a test cup, reading the transmittance data of the platelet-rich plasma sample within a specified time in the formation process of platelet aggregation; imbibing a strong inducer or a composite inducer, injecting into a test cup, conducting continuous detection on the test cup by a platelet aggregation test device; when all platelets in the platelet-rich plasma nearly reach the aggregation state and the maximum transmittance is reached, automatically reading the transmittance data; and calculating the maximum aggregation rate of the platelet of the measured blood sample, and generating a test report according to the input patient information.

Description

technical field [0001] The invention relates to a method for measuring platelet aggregation rate. The platelet aggregation rate is measured according to photoelectric turbidimetry, and a single platelet-rich plasma is used to realize photoelectric turbidimetry (abbreviated as LTA) to measure the platelet aggregation rate. Background technique [0002] Chinese patent 200910083611.6 discloses a method for measuring platelet aggregation. An automatic platelet aggregator is used for measurement based on photoelectric turbidimetry. The measurement steps are: preparing platelet aggregation inducer, prepared platelet-rich plasma and platelet-poor plasma Place it in the sample tray device, order the sampling needle to absorb platelet-poor plasma, inject it into the test cup of the test mechanism device, and read the transmittance data of the platelet-poor plasma sample; order the sampling needle to absorb platelet-rich plasma, and inject it into the test mechanism In the test cup of...

Claims

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Application Information

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IPC IPC(8): G01N21/59
Inventor 陈生王建中邱焕波王顺义宫宇山肖继昌
Owner 山东泰利信医疗科技有限公司
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