LRRK2 gene 1628 polymorphism detection kit

A technology of polymorphism detection and kit, which is applied in the determination/testing of microorganisms, DNA/RNA fragments, recombinant DNA technology, etc., to achieve the effect of increasing throughput, improving accuracy, and rapid detection

Inactive Publication Date: 2016-09-21
XIAMEN RENRUI BIOMEDICAL TECH CO LTD
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  • Abstract
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AI Technical Summary

Problems solved by technology

At present, there is no report on the study of the 1628 polymorphism of the LRRK2 gene using real-time fluorescent PCR

Method used

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  • LRRK2 gene 1628 polymorphism detection kit
  • LRRK2 gene 1628 polymorphism detection kit
  • LRRK2 gene 1628 polymorphism detection kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1 Real-time fluorescent PCR detection of three types of LRRK2 1628 polymorphic sites (rs33949390).

[0027] 1. Materials

[0028] 1. Instruments: real-time fluorescent PCR instrument, pipette, centrifuge.

[0029] 2. Design of primers and probes: The present invention designs primers that can specifically amplify the DNA fragment containing the 1628 polymorphic site of the LRRK2 gene, and a probe that specifically recognizes the 1628 polymorphic site of the LRRK2 gene. LRRK2 gene 1628 polymorphism site (rs33949390) c.4883 base is G labeled as FAM, LRRK2 gene 1628 polymorphic site (rs33949390) c.4883 base is C labeled as HEX. Therefore, in the reaction tube where the c.4883 base is G / G sample as the template, the FAM channel should have an amplification signal, and the HEX channel should have no amplification signal; the c.4883 base is the C / C sample as the template. In the reaction tube, the HEX channel should have amplification signals, and the FAM channel sh...

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Abstract

The invention relates to an LRRK2 gene 1628 polymorphism detection kit, relating to gene polymorphism detection. The LRRK2 gene 1628 polymorphism detection kit comprises primers, fluorescent probes and reagents, wherein the primers are used for carrying out specific amplification on a DNA fragment containing the 1628 site of the LRRK2 gene, and the base sequences of the primers are shown as SEQ ID NO:1 and SEQ ID NO:2; the fluorescent probes are used for recognizing the polymorphism of the LRRK2 gene 1628, the 5' end of each fluorescent probe marks the fluorophore, the 3' end of each fluorescent probe marks the cancellation group; two nucleic acid probes used for marking two different fluorophore marks are adopted for simultaneously indicating two polymorphism conditions in one reaction tube, and the base sequences are shown as SEQ ID NO:3 and SEQ ID NO:4; the reagents comprise MgCl2, warm start Taq polymerase, Uracil-N-Glycosylase, dATP, dCTP, dGTP, dTTP, dUTP and PCR reaction buffer.

Description

technical field [0001] The invention relates to gene polymorphism detection, in particular to a LRRK2 gene 1628 polymorphism detection kit. Background technique [0002] Parkinson's disease (PD) is the second most common neurodegenerative disorder after Alzheimer's disease (AD). The prevalence of PD among people over 65 years old in my country is 1.17%, which is similar to developed countries in Europe and the United States. This ratio rises to 3% among people over 75 years old. Therefore, Parkinson's disease has become a serious threat to human health in modern society in my country. one of the major diseases. [0003] The average age of Parkinson's disease diagnosis is 70 years old, but its symptoms appear earlier than the clinical diagnosis. Therefore, finding early biological markers of Parkinson's disease and performing genetic diagnosis are extremely important for its risk prediction and later prevention. Parkinson's disease usually lasts 15 years from diagnosis to de...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6883C12Q1/6858C12Q2600/156C12Q2531/113C12Q2563/107C12Q2561/113
Inventor 郭奇伟谢勇壮钟力许华曦卜国军曹甜甜
Owner XIAMEN RENRUI BIOMEDICAL TECH CO LTD
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