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Antimicrobial and recyclable cell capture device and recycling method thereof

A technology for capturing devices and cells, applied in the field of modern medicine, can solve the problems of low specificity, waste, and low purity of size-sorted CTCs, and achieve the effects of wide application prospects, market space, and strong antibacterial ability.

Active Publication Date: 2019-08-16
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The size of cancer cells cultured in vitro is indeed significantly different from that of blood cells in vivo, but it has been reported that the size of CTCs entering the blood system is very different from that of the same type of cancer cells cultured in vitro, which is closer to the size of blood cells in vivo and depends on the size The specificity of sorting CTC is not high, which will lead to low purity; at present, many capture devices lack the means of collecting and enriching cells and aseptic protection. At the same time, most of the devices are disposable and cannot be reused, resulting in high cost and waste

Method used

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  • Antimicrobial and recyclable cell capture device and recycling method thereof
  • Antimicrobial and recyclable cell capture device and recycling method thereof
  • Antimicrobial and recyclable cell capture device and recycling method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment one, the synthesis of the PDMS substrate with multiple pillars

[0031] First, design the 3D structure with AutoCAD, and make a silicon wafer mold with the designed structure. The PDMS prepolymer and curing agent are mixed at a mass ratio of 10:1 and then poured on the mold, vacuumed by a vacuum pump, heated on a 90°C heating plate for 2 hours, and can be used after being peeled off from the mold. Such as figure 1 b and 1c show the PDMS substrate with cylinders.

Embodiment 2

[0032] Embodiment two, the synthesis of zinc oxide nanomaterial

[0033] Using the method of magnetron sputtering at 120°C for 30 minutes, a layer of 400-600nm seed crystal layer was grown on the clean PDMS substrate. Next, the growth solution was prepared. The growth solution was composed of zinc nitrate hexahydrate and urethane In the mixed solution composed of tropine, the final concentration of the two compounds was 10 mM. Soak the PDMS plate with the seed layer in the growth solution and incubate on a 90°C heating plate for 48 hours. Such as figure 1 d and 1e are ZnO nanomaterials grown on PDMS substrates.

Embodiment 3

[0034] Embodiment 3, the activation of the capture device.

[0035] After the nano-zinc oxide material is grown, the device obtained in Example 2 is subjected to plasma etching for 1 minute. Afterwards, the device was soaked in 4% (v / v) (3-aminopropyl)trimethoxysilane in ethanol and incubated at room temperature for 1 hour. After cleaning, soak the device in 10 mM N,N'-disuccinimidyl carbonate (DSC) solution in acetonitrile, incubate at room temperature for 10 minutes, and clean. Then, the device was added with 10 μg / mL neutravidin prepared in phosphate buffered solution (PBS), incubated at room temperature for 60 minutes, and washed. Then 10 μg / mL biotin-linked anti-EpCAM or anti-vimentin antibody diluted with 1% BSA in PBS was added. Incubate at room temperature for 1 hour and wash. For each wash, the device was soaked in PBS solution and placed on a shaker for 10 min. Finally, 5% BSA solution prepared in PBS was used to block for 1 hour at room temperature to reduce non...

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Abstract

The invention discloses an antibacterial recyclable cell capturing device and a circulation method thereof. The antibacterial recyclable cell capturing device consists of a substrate and a nano zinc oxide material. After being treated with a weak acid solution, nano zinc oxide can effectively capture and release cells harmlessly, and meanwhile a retained zinc oxide material can be activated to capture and release cells of a next round. By virtue of the device structure disclosed by the invention, capturing and release of circulating tumor cells (CTCs) can be integrated, the purpose of circulation utilization is achieved, bacterial infection is avoided in the capturing process, the limit of mere physical counting on CTCs in a conventional method strategy is avoided, requirements of use environments and sterile treatment are met, biological analysis on CTCs is achieved for operators, and a very good technical platform is provided for early-stage diagnosis, accompany diagnosis and individual treatment on cancer patients in clinical treatment.

Description

technical field [0001] The invention belongs to modern medical technology, and in particular relates to a cell or microorganism sorting device, in particular to a biological sorting system for sorting and enriching tumor cells and a manufacturing process thereof. Background technique [0002] Cancer is the number one cause of death in developed countries and the second in developing countries. Clinically speaking, the main reason for the high mortality of cancer patients is the delay of disease diagnosis and inappropriate treatment plan. Physiologically, most of the deaths caused by cancer are caused by the migration of tumor cells to other organs in the body through the hematogenous route. In this process, metastatic cancer cells, namely circulating tumor cells (CTCs), play a key role in cancer metastasis. Accumulating evidence indicates that the metastasis of tumor cells from the primary site to other sites occurs early in cancer development. Therefore, on the one hand,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/00C12N5/09
CPCC12M47/04C12N5/0693C12N2509/00
Inventor 王本惠兰兰苏逸
Owner ZHEJIANG UNIV
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