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Kit for predicting curative effect of glucocorticoids on curing SLE (Systemic Lupus Erythematosus) on basis of HSP90AB1 genotype

A technology of glucocorticoids and genotypes, applied in the field of medicine and health

Active Publication Date: 2016-09-21
ANHUI MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is a lack of methods and kits for predicting the curative effect of GC in treating SLE based on the genotype of HSP90AB1 gene polymorphism

Method used

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  • Kit for predicting curative effect of glucocorticoids on curing SLE (Systemic Lupus Erythematosus) on basis of HSP90AB1 genotype
  • Kit for predicting curative effect of glucocorticoids on curing SLE (Systemic Lupus Erythematosus) on basis of HSP90AB1 genotype
  • Kit for predicting curative effect of glucocorticoids on curing SLE (Systemic Lupus Erythematosus) on basis of HSP90AB1 genotype

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Embodiment 1

[0043]Example 1: A kit and method for predicting the efficacy of glucocorticoids in treating SLE based on the genotype of the HSP90AB1 gene polymorphism

[0044] (1) Components of the kit

[0045] (1) Primers for detecting the genotype of the HSP90AB1 gene polymorphism site;

[0046] Primers for amplifying polymorphic loci:

[0047]

[0048] Primers to extend polymorphic sites:

[0049]

[0050] (2) PCR amplification enzyme and corresponding buffer;

[0051] (3) PCR product purification enzyme and corresponding buffer;

[0052] (4) dNTPs;

[0053] (5) Single base extension reaction enzyme and corresponding buffer;

[0054] (6) Single-base extension product purification enzyme and corresponding buffer.

[0055] (2) Specimen collection preparation and genotype detection

[0056] 1 Collect blood samples

[0057] Professional medical staff collected 5ml of venous blood from the forearm of SLE patients diagnosed according to the SLE classification criteria of the Amer...

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Abstract

The invention relates to a kit for predicting the curative effect of glucocorticoids on curing SLE (Systemic Lupus Erythematosus) on basis of an HSP90AB1 genotype. The kit comprises the components: a primer for detecting the HSP90AB1 gene polymorphism site genotype, a PCR (Polymerase Chain Reaction) amplification enzyme and a corresponding buffer solution, a PCR product purification enzyme, dNTP (Diethyl-nitrophenyl Thiophosphate), a single-base extension reaction enzyme and a corresponding buffer solution, and a single-base extension product purification enzyme. When the HSP90AB1 gene rs9367190 polymorphism site genotype is CA and / or AA, the curative effect of the glucocorticoids on curing the SLE (Systemic Lupus Erythematosus) is predicted to be good. When a haplotype formed by rs9367190 and rs13296 polymorphism sites is CA, the curative effect of the glucocorticoids on curing the SLE is predicted to be poor. When an rs13296 polymorphism site genotype is GA and / or AA, the living quality (the mental health) improvement of an SLE patient cured by the glucocorticoids is predicted to be poor. The invention provides a new idea and a new method for guiding individualized treatment of SLE patients.

Description

technical field [0001] The invention relates to a kit and method for predicting the curative effect of glucocorticoid in treating SLE based on the genotype of HSP90AB1 gene polymorphism site, belonging to the technical field of medicine and health. Background technique [0002] Systemic lupus erythematosus (SLE) is a common autoimmune disease involving multiple systems and organs. There is a gender difference in the prevalence of SLE. It occurs more frequently in young and middle-aged women, but it is also seen in children and the elderly. The prevalence ratio of male to female is about 1:>8. At present, the pathogenesis of SLE is not fully understood, and evidence shows that genetic factors and environmental factors are the two main factors leading to its occurrence. The incidence rate of close relatives of SLE patients is about 5%, and the incidence rate of identical twins (about 68%) is much higher than that of fraternal twins (about 3%). Epidemiological surveys show...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
CPCC12Q1/686C12Q1/6883C12Q2600/156
Inventor 邹延峰叶冬青徐建华潘发明谷园园孙秀秀
Owner ANHUI MEDICAL UNIV
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