Application of Chinese wistaria extract to preparation of health food capable of relieving physical fatigue
A technology for relieving physical fatigue, extracts, applied in the food field, can solve problems such as slow brain response and human health hazards, and achieve the effect of relieving physical fatigue
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Embodiment 1
[0014] Example 1: Preparation of Wisteria Extract 1
[0015] (a) Mix 5kg of dried wisteria and licorice (accounting for 10% of the total weight), crush them, extract with 75% ethanol solution under heat reflux (20L x 3 times), combine the extracts, concentrate until there is no alcohol smell to obtain an ethanol extraction concentrate (b) Dilute the ethanol extraction concentrate obtained in step (a) with water (3L), and extract with petroleum ether (3L×3 times), ethyl acetate (3L×3 times) and water-saturated n-butanol (3L ×3 times), concentrated under reduced pressure to obtain petroleum ether extract, ethyl acetate extract and n-butanol extract respectively; (c) n-butanol extract was dissolved in water, filtered, and enriched with AB-8 macroporous resin For active ingredients, wash 8 column volumes with 15% ethanol to remove large polar components, then elute 10 column volumes with 55% ethanol, collect the 55% ethanol eluate, and concentrate under reduced pressure.
Embodiment 2
[0016] Embodiment 2: Preparation of wisteria extract 2
[0017] (a) Mix 5kg of dried wisteria and licorice (accounting for 5% of the total weight), pulverize, extract with 70% ethanol solution under heat reflux (20L x 3 times), combine the extracts, concentrate until there is no alcohol smell to obtain an ethanol extraction concentrate (b) Dilute the ethanol extraction concentrate obtained in step (a) with water (3L), and extract with petroleum ether (3L×3 times), ethyl acetate (3L×3 times) and water-saturated n-butanol (3L ×3 times), concentrated under reduced pressure to obtain petroleum ether extract, ethyl acetate extract and n-butanol extract respectively; (c) n-butanol extract was dissolved in water, filtered, and enriched with AB-8 macroporous resin For active ingredients, wash 10 column volumes with 10% ethanol to remove large polar components, then elute 12 column volumes with 50% ethanol, collect the 50% ethanol eluate, and concentrate under reduced pressure.
Embodiment 3
[0018] Embodiment 3: the preparation of wisteria extract
[0019] (a) Mix 5kg of dried wisteria and licorice (accounting for 15% of the total weight), crush them, extract them under reflux with 80% ethanol solution, combine the extracts (20L x 3 times), concentrate until there is no alcohol smell to obtain an ethanol extraction concentrate ; (b) Dilute the ethanol extraction concentrate obtained in step (a) with water (3L), and then use petroleum ether (3L×3 times), ethyl acetate (3L×3 times) and water-saturated n-butanol (3L×3 times) 3 times) extraction, concentration under reduced pressure to obtain petroleum ether extract, ethyl acetate extract and n-butanol extract respectively; (c) n-butanol extract was dissolved in water, filtered, enriched with AB-8 macroporous resin For the active ingredient, wash 6 column volumes with 20% ethanol to remove large polar components, then elute 8 column volumes with 60% ethanol, collect the 60% ethanol eluate, and concentrate under reduce...
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