Deep sea microorganism in-situ cultivation and enrichment device

Abstract

The invention discloses a deep sea microorganism in-situ cultivation and enrichment device. The device comprises a six-side box which can be freely disassembled and assembled. The six-side box is composed of a front plate, a rear plate, a bottom plate, a top plate, a left plate and a right plate. Through holes are formed in the front plate, the rear plate, the bottom plate, the top plate, the left plate and the right plate. In the six-side box, grooves are correspondingly formed in the parts, between every two adjacent rows of through holes, on the inner walls of the front plate and the rear plate. A microorganism attaching base plate is arranged between every two opposite grooves and from the top plate to the bottom plate in an inserted mode. The internal space of the six-side box is divided into a plurality of sub-spaces by the microorganism attaching base plates. Fasteners are arranged in the corresponding through holes in the front plate and the rear plate and in the corresponding through holes in the bottom plate and the top plate in a penetrating mode respectively. The deep sea microorganism in-situ cultivation and enrichment device has the advantages that the six-side box has higher pressure-withstanding capability and can be used for hadal or super-hadal microorganism in-situ cultivation and enrichment; by means of the microorganism attaching base plates made of different materials, microorganisms can be easily and comprehensively enriched under extreme conditions, and the natural state of the microflora structure can be maintained; the deep sea microorganism in-situ cultivation and enrichment device is easy to manufacture, low in manufacturing cost and capable of saving experiment cost.

Description

technical field [0001] The invention belongs to the technical field of marine microorganism cultivation, and in particular relates to a device for in-situ cultivation and enrichment of deep sea microorganisms. Background technique [0002] The cultivation of microorganisms plays an irreplaceable role in the basic research and application development of microorganisms and their products. However, the vast majority of microorganisms currently belong to "uncultured microorganisms" that cannot be cultured in the laboratory. According to the analysis, the proportion of uncultivated microorganisms in terrestrial microorganisms (including fresh water, soil, sludge, etc.) is about 85-99.75%, while the proportion of uncultivated microorganisms in the ocean is as high as 99.9-99.9999%. With the continuous deepening of people's understanding of the unique and important role of microorganisms in the global biogeochemical cycle of ecosystems and resource development and utilization, it ...

AI Technical Summary

Problems solved by technology

Although these technologies can transfer samples collected in deep sea or super abyss in parallel under pressure, there are still various shortcomings. On the one hand, the cost is expensive, costing millions of dollars; on the other hand, limited by cost, Due to factors such as materials and quality, the volume of the culture vessel is too low (≈10 mL), so its application range is extremely limited, and it cannot be used in ecological environment and resource investigations such as microbial population and community structure analysis.

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