Microbial plate culture method

A technology of plate culture and plate culture medium, applied to bacteria and other directions, can solve the problems of inability to count colonies, high plate requirements, and complicated culture process, and achieve the effects of easy observation, reduced requirements, and simple operation.

Inactive Publication Date: 2016-10-12
郑州点石生物技术有限公司
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0007] Although the above two methods can achieve the purpose of observing the characteristics of the colony, the plate streak separation method cannot count the colonies, and the dilution coating plate method has a complicated cultivation process and relatively high requirements for the plate

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  • Microbial plate culture method

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Embodiment Construction

[0023] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention.

[0024] Such as figure 1 Shown, microorganism plate culture method of the present invention, comprises the following steps:

[0025] a. Preparation of plate culture medium

[0026] Heat the sodium chloride sucrose agar medium to melt and cool to 65°C, add 3-5ml of clindamycin solution to the sodium chloride sucrose agar medium and mix well, then take 20ml of the well-mixed medium solution and pour it into the culture medium Gently shake the culture dish to distribute the culture medium solution evenly on the bottom of the culture dish. After the culture medium solution is solidified, the plate culture medium is obtained.

[0027] b. Preparation of activated sludge m...

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Abstract

The invention discloses a microbial plate culture method. The microbial plate culture method comprises the steps of plate culture medium preparation, active sludge mixed liquor preparation, culture medium coating, bacterial colony culture and bacterial colony separation. The microbial plate culture method is easy to operate, has no requirement on colonial morphology of bacteria, and is convenient for observing colony characteristics and colony counting; by adopting the method, the requirement on the plate culture medium is lowered, and production and scientific research work are ensured.

Description

technical field [0001] The invention relates to the technical field of microorganism cultivation, in particular to a method for culturing microorganisms on a flat plate. Background technique [0002] In order to observe and study the condition of colonies, the following two microbial culture methods are usually used at present: [0003] 1. Flat scribe separation method [0004] Microorganisms mixed together or different cells in the same microbial population are circled on the surface of the plate medium with an inoculation ring, and more independently distributed single cells are obtained by dividing and diluting, and grow and reproduce into a single colony after culture. [0005] 2. Dilution coating plate method [0006] Do a series of gradient dilutions of the bacterial solution, and then apply different dilutions of the bacterial solution to the surface of the agar solid medium for cultivation. In the sufficiently high dilution of the bacterial solution, the aggregated...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20
CPCC12N1/20
Inventor 李杰
Owner 郑州点石生物技术有限公司
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