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Kit for detecting gastric cancer related NDRG4 gene methylation degree and application thereof

A methylation and kit technology, which is applied in the field of kits for the methylation degree of NDRG4 gene, can solve the problem that there is no public detection kit for detecting the methylation degree of the CpG island at the 3' end of the NDRG4 gene, etc., and achieve the detection result. Accurate, reliable, targeted and sensitive effects

Active Publication Date: 2016-10-12
NINGBO UNIV
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Problems solved by technology

At present, there are no relevant research reports on the use of pyrosequencing to detect the degree of methylation of the CpG island at the 3' end of the NDRG4 gene in gastric cancer.

Method used

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  • Kit for detecting gastric cancer related NDRG4 gene methylation degree and application thereof
  • Kit for detecting gastric cancer related NDRG4 gene methylation degree and application thereof
  • Kit for detecting gastric cancer related NDRG4 gene methylation degree and application thereof

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Embodiment Construction

[0019] The present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments.

[0020] 1. Collection of research objects and samples

[0021] A total of 110 gastric cancer tissues and corresponding paired adjacent normal tissues were collected from a hospital in Hangzhou City, Zhejiang Province from December 2007 to February 2010. The cancer tissues were taken from the center of the tumor tissue, and the distance between the adjacent tissues was The cancerous tissue was more than 5cm and was non-cancerous tissue. All cases had not undergone radiotherapy and chemotherapy before operation and had been confirmed by histopathological examination. Patients aged 21-83 years, including 76 males and 34 females; 30 cases with smoking history, 80 cases without smoking history; 28 cases with drinking history, 82 cases without drinking history; tumor size: ≥6cm 43 cases, <6cm 67 cases. According to the American Joint Committee on C...

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Abstract

The invention discloses a kit for detecting the gastric cancer related NDRG4 gene methylation degree and an application thereof. The kit is characterized by a pair of NDRG4 gene methylation specific amplification primers and a methylation specific sequencing primer, wherein the nucleotide sequence of the forward primer is 5'-AGGGTTGGGGGTTTTAGA-3'; the nucleotide sequence of the reverse primer is 5'-Biotin-CACCCTCTACCAAAAACTCAAAACTCAATT-3'; and the nucleotide sequence of the sequencing primer is 5'-GGGGTTTTAGAGTGTAT-3'. The kit has the advantages of easiness and convenience in detection, strong targeting property and high detection accuracy and efficiency and is used for preparing a reagent or kit for early diagnosis of gastric cancer.

Description

technical field [0001] The invention relates to a method for assisting early diagnosis of gastric cancer, in particular to a kit for detecting the methylation degree of NDRG4 gene related to gastric cancer and its application. Background technique [0002] Gastric cancer is a malignant tumor originating from the gastric epithelium and is one of the most common malignant tumors worldwide. Its morbidity and fatality rate ranks fourth and third in the world, and it is a disease that seriously threatens human life and health. In China, the incidence rate of gastric cancer is 29.9 / 100,000, and the death rate is 22.3 / 100,000. Compared with other countries in the world, among the male and female gastric cancer population in China, the cancer mortality rate ranks first in the world. With the development of medical methods and the popularization of health concepts, although the incidence and mortality of gastric cancer are declining, the overall prognosis is still poor. Due to the...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
CPCC12Q1/6886C12Q2600/118C12Q2600/154
Inventor 段世伟陈晓颖
Owner NINGBO UNIV
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