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Kit for detection of ndrg4 gene methylation for early diagnosis and prognosis evaluation of gastric cancer and its application

A detection kit and methylation technology, which is applied in the field of NDRG4 gene methylation detection for early diagnosis and prognosis evaluation of gastric cancer, can solve the problems of non-disclosure, achieve high sensitivity and specificity, high sensitivity, improve sensitivity and specificity sexual effect

Active Publication Date: 2020-01-07
宁波思锐生物科技有限公司
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Problems solved by technology

At present, at present, there are no relevant research reports on the test kit for detecting the methylation degree of the NDRG4 gene promoter region to assess the risk and overall prognosis of gastric cancer at home and abroad.

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  • Kit for detection of ndrg4 gene methylation for early diagnosis and prognosis evaluation of gastric cancer and its application
  • Kit for detection of ndrg4 gene methylation for early diagnosis and prognosis evaluation of gastric cancer and its application
  • Kit for detection of ndrg4 gene methylation for early diagnosis and prognosis evaluation of gastric cancer and its application

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Embodiment Construction

[0030] The present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments.

[0031] 1. Collection of research objects and samples

[0032] A total of 110 gastric cancer tissues and corresponding paired adjacent normal tissues were collected from a hospital in Hangzhou City, Zhejiang Province from December 2007 to February 2010. The cancer tissues were taken from the center of the tumor tissue, and the distance between the adjacent tissues was The cancerous tissue was more than 5cm and was non-cancerous tissue. All cases had not undergone radiotherapy and chemotherapy before operation and had been confirmed by histopathological examination. The patients were aged from 21 to 83 years, including 76 males and 34 females; 30 cases had smoking history, 80 cases had no smoking history; 28 cases had drinking history, 82 cases had no drinking history; tumor size: ≥6cm, 43 cases, <6cm 67 cases. According to the American Joint ...

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Abstract

The invention discloses an NDRG4 (N-myc Downstream-Regulated Gene 4) gene methylation detection kit for early diagnosis and prognosis evaluation of gastric cancer. The NDRG4 gene methylation detection kit is characterized by comprising a pair of NDRG4 gene promoter region methylation specific amplification primers and a fluorescence quantitative probe; specific nucleotide sequences are as follows: an upstream primer: 5'-TGGGATGGGGATGTTTTTGT-3'; a downstream primer: 5'-CCTCCAAACCCCCTATAACC-3'; the fluorescence quantitative probe: 5'-6-FAM-AAAACGACGCTACCGTAATCTTTA-BHQ1-3'. The NDRG4 gene methylation detection kit has the advantages of simplicity and convenience for operation, short period, high sensitivity, good specificity and high detection accuracy.

Description

technical field [0001] The invention relates to the technical field of disease-related DNA detection, in particular to a kit for detecting methylation of NDRG4 gene for early diagnosis and prognosis evaluation of gastric cancer and its application. Background technique [0002] Gastric cancer is one of the most common malignant tumors that seriously endanger human health. Its incidence rate ranks second among all malignant tumors, and its mortality rate ranks third among all malignant tumors. At present, radical surgery is still the main treatment for gastric cancer, and it is also the only hopeful way to cure gastric cancer. However, clinically, most patients are diagnosed in the middle and advanced stages, and the surgical effect is not good, which directly affects the prognosis. Therefore, in-depth study of the molecular mechanism of gastric cancer occurrence and development can provide a basis for early diagnosis of gastric cancer and provide effective target therapy fo...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6886
CPCC12Q1/6886C12Q2600/112C12Q2600/118C12Q2600/154
Inventor 段世伟施长根陈晓颖
Owner 宁波思锐生物科技有限公司
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