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NDRG4 (N-myc Downstream-Regulated Gene 4) gene methylation detection kit for early diagnosis and prognosis evaluation of gastric cancer and application of NDRG4 gene methylation detection kit

A technology for early diagnosis and prognosis evaluation, applied in the direction of microbial determination/examination, biochemical equipment and methods, etc., can solve the problem of non-disclosure, etc., achieve high sensitivity and specificity, improve sensitivity and specificity, and high sensitivity.

Active Publication Date: 2016-11-16
宁波思锐生物科技有限公司
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Problems solved by technology

At present, at present, there are no relevant research reports on the test kit for detecting the methylation degree of the NDRG4 gene promoter region to assess the risk and overall prognosis of gastric cancer at home and abroad.

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  • NDRG4 (N-myc Downstream-Regulated Gene 4) gene methylation detection kit for early diagnosis and prognosis evaluation of gastric cancer and application of NDRG4 gene methylation detection kit
  • NDRG4 (N-myc Downstream-Regulated Gene 4) gene methylation detection kit for early diagnosis and prognosis evaluation of gastric cancer and application of NDRG4 gene methylation detection kit
  • NDRG4 (N-myc Downstream-Regulated Gene 4) gene methylation detection kit for early diagnosis and prognosis evaluation of gastric cancer and application of NDRG4 gene methylation detection kit

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Embodiment Construction

[0030] The present invention will be further described in detail below with reference to the embodiments of the accompanying drawings.

[0031] 1. Collection of research objects and samples

[0032] A total of 110 gastric cancer tissues and corresponding matched adjacent normal tissues were collected from a hospital in Hangzhou City, Zhejiang Province from December 2007 to February 2010. The cancer tissues were taken from the center of the tumor tissue and the distance between the adjacent tissues The cancer tissue was more than 5 cm and was non-cancerous tissue. All cases had not received radiotherapy and chemotherapy before surgery and were confirmed by histopathological examination. The age of the patients ranged from 21 to 83 years, including 76 males and 34 females; 30 patients had a history of smoking and 80 patients had no history of smoking; 28 patients had a history of drinking and 82 patients had no history of drinking; tumor size: ≥6cm in 43 patients, <6cm 67 cases...

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Abstract

The invention discloses an NDRG4 (N-myc Downstream-Regulated Gene 4) gene methylation detection kit for early diagnosis and prognosis evaluation of gastric cancer. The NDRG4 gene methylation detection kit is characterized by comprising a pair of NDRG4 gene promoter region methylation specific amplification primers and a fluorescence quantitative probe; specific nucleotide sequences are as follows: an upstream primer: 5'-TGGGATGGGGATGTTTTTGT-3'; a downstream primer: 5'-CCTCCAAACCCCCTATAACC-3'; the fluorescence quantitative probe: 5'-6-FAM-AAAACGACGCTACCGTAATCTTTA-BHQ1-3'. The NDRG4 gene methylation detection kit has the advantages of simplicity and convenience for operation, short period, high sensitivity, good specificity and high detection accuracy.

Description

technical field [0001] The invention relates to the technical field of disease-related DNA detection, and more particularly to a kit for detecting NDRG4 gene methylation used for early diagnosis and prognosis evaluation of gastric cancer and its application. Background technique [0002] Gastric cancer is one of the most common malignant tumors that seriously endanger human health, and its incidence ranks second among all malignant tumors, and its mortality ranks third among all malignant tumors. At present, radical surgery is still the main treatment for gastric cancer, and it is also the only promising method for curing gastric cancer. However, most of the patients are still in the middle and late stages when they are diagnosed clinically, and the surgical effect is not good, which directly affects the prognosis. Therefore, in-depth study of the molecular mechanism of gastric cancer occurrence and development can provide a basis for early diagnosis of gastric cancer, and ...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/6886C12Q2600/112C12Q2600/118C12Q2600/154
Inventor 段世伟施长根陈晓颖
Owner 宁波思锐生物科技有限公司
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