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Urease biosensor based on polyaniline-modified screen-printed electrode and its application

A technology of screen-printed electrodes and biosensors, which is applied in the field of analysis and detection, can solve the problems of time-saving, labor-saving, and low-cost, and achieve the effects of preventing enzyme leakage, good reproducibility, and maintaining activity

Inactive Publication Date: 2018-07-31
江苏出入境检验检疫局工业产品检测中心 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Although the existing analysis methods have good precision and high accuracy, the existing analysis methods cannot meet the requirements of time saving, labor saving, cheapness, reduction of solvents, and reduction of environmental pollution, etc.

Method used

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  • Urease biosensor based on polyaniline-modified screen-printed electrode and its application
  • Urease biosensor based on polyaniline-modified screen-printed electrode and its application
  • Urease biosensor based on polyaniline-modified screen-printed electrode and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Electropolymerization of polyaniline with screen-printed electrodes: first, place the screen-printed electrodes in an electropolymerization solution containing hydrochloric acid and aniline, wherein the molar concentration of HC1 is 1 mol / L, and the molar concentration of aniline is 0.5 mol / L; then use The electropolymerization was carried out by cyclic voltammetry. The scanning range of cyclic voltammetry was -0.1V~0.7V, and the scanning rate was 50mV / s. A total of 100 scans were performed. Before the start of the experiment, the electropolymerization solution needs to pass nitrogen gas for 10 minutes, and the whole electropolymerization process is completed under nitrogen atmosphere. After the electropolymerization is completed, the electrode is rinsed with 1mol / L hydrochloric acid and secondary water, and dried to obtain a PAIN modified electrode;

[0036] Platinum / carbon sphere nanocomposite material: Add 80mg of carbon spheres to ethylene glycol solution and disper...

Embodiment 2

[0039]Screen-printed electrode electropolymerized polyaniline: first place the screen-printed electrode in an electropolymerization solution containing hydrochloric acid and aniline, in which the molar concentration of HC1 is 2 mol / L, and the molar concentration of aniline is 1 mol / L; Electropolymerization was carried out by voltammetry. The scanning range of cyclic voltammetry was -0.1V~0.7V, and the scanning rate was 40mV / s. A total of 80 scans were performed. Before the start of the experiment, the electropolymerization solution needs to pass nitrogen gas for 10 minutes, and the whole electropolymerization process is completed under nitrogen atmosphere. After the electropolymerization is completed, the electrode is rinsed with 1mol / L hydrochloric acid and secondary water, and dried to obtain a PAIN modified electrode;

[0040] Platinum / carbon sphere nanocomposite material: Add 50mg of carbon spheres to the ethylene glycol solution and disperse it uniformly by ultrasonic to ...

Embodiment 3

[0043] Screen-printed electrode electropolymerized polyaniline: first place the screen-printed electrode in an electropolymerization solution containing hydrochloric acid and aniline, wherein the molar concentration of HC1 is 3 mol / L, and the molar concentration of aniline is 1.5 mol / L; then use The electropolymerization was carried out by cyclic voltammetry. The scanning range of cyclic voltammetry was -0.1V~0.7V, and the scanning rate was 60mV / s. A total of 120 scans were performed. Before the start of the experiment, the electropolymerization solution needs to pass nitrogen gas for 10 minutes, and the whole electropolymerization process is completed under nitrogen atmosphere. After the electropolymerization is completed, the electrode is rinsed with 1mol / L hydrochloric acid and secondary water, and dried to obtain a PAIN modified electrode;

[0044] Platinum / carbon sphere nanocomposite material: Add 120mg carbon spheres to ethylene glycol solution and disperse uniformly by ...

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Abstract

The invention discloses a urease biosensor based on a polyaniline-modified screen-printed electrode and application of the urease biosensor, belonging to the field of analysis and detection. According to the biosensor disclosed by the invention, a biosensor based on a polyaniline film and chitosan / platinum-carbon pellets is constructed by using a screen-printed electrode, and rapid detection on heavy metal ions is realized. The urease biosensor prepared according to the technical solution has favorable reproducibility, is time-saving, labor-saving and low in cost, reduces a solvent, reduces the pollution to the environment, and the like. The biosensor can be stored in a refrigerator at 4 DEG C when not in use, a potential response of the biosensor within three weeks cannot drop, and 95 percent of initial potential response can be still kept after the biosensor is stored for 30 days, which indicates that chitosan can effectively keep the activity of urease, and prevent urease leakage.

Description

technical field [0001] The invention belongs to the field of analysis and detection, in particular to a urease biosensor based on a polyaniline-modified screen-printed electrode and an application thereof. Background technique [0002] With the popularization of the concept of green consumption, ecological textiles have become more and more mainstream in the market. In recent years, the detection of extractable heavy metals in textiles has been paid more and more attention as one of the indicators of ecological textiles. Heavy metals in textiles mainly come from dyes and auxiliaries used in the processing process, such as various metal complex dyes, medium fuels, phthalocyanine structure dyes, color fixing agents, catalysts, flame retardants, finishing agents, etc. Various metal complexing agents in soft water hardening, desizing and scouring, bleaching and printing processes. The extractable heavy metals in textiles will enter the skin of the human body through the sweat o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/26G01N27/30
CPCG01N27/26G01N27/308G01N27/3278
Inventor 吴丽娜曹锡忠周静洁毛欣周静珠
Owner 江苏出入境检验检疫局工业产品检测中心