Methods of Improving Fish's Ability to Tolerate Low Temperature
A fish and low-temperature technology, applied in the field of economic fish and ornamental fish species improvement, can solve the problems of high technical equipment requirements, long time required, low efficiency, etc., achieve wide application value, less safety and ethical problems, and reduce The effect of farming temperature
Inactive Publication Date: 2018-09-28
SHANGHAI OCEAN UNIV
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It also has disadvantages such as low efficiency, long time required or high technical equipment requirements, and it is difficult to quickly be widely used in some small producers and uncommon fish
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[0024] In this example, zebrafish were used as the experimental material, and the heat shock protein HSP90 inhibitor (Radicicol, radicicol) was given for 24 hours during the development of fish fertilized eggs, and the fish individuals that developed subsequently could appear individuals with enhanced low temperature resistance . These individuals continue to undergo the same treatment process, and the low temperature resistance can be further improved to a certain extent. Its ability to tolerate low temperatures can be passed on to offspring.
[0025] The specific steps of the method for improving the low temperature tolerance of fish of the present embodiment are as follows:
[0026] (1) Preparation of Radicicol stock solution: Dissolve Radicicol in dimethyl sulfoxide (DMSO) to prepare a stock solution with a Radicicol concentration of 25 mM, and store it at minus 20 degrees Celsius. Melt at room temperature when used, and further dilute with fish egg incubation water to a...
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The invention discloses a method for improving fish low temperature tolerance. The method comprises the following contents: radicicol is applied during a fish fertilized egg development stage for incubation; incubation water for incubation is a dimethyl sulfoxide solution with 5-10muM radicicol; and the fish fertilized egg development stage is from a fish fertilized egg development cell stage to 50% coated stage. With the method provided by the invention, fish low temperature resistance can be rapidly and conveniently improved. The method has a good application value. Compared to natural individual screening and hybridization, the speed of the method is higher. Compared to a transgenic method, the method provided by the invention is easier to implement, and has lower cost and less safety and ethical issues. In the aspect of application, first, low temperature resistance of economic aquaculture fish can be improved, such that loss caused by low-temperature weather can be reduced; second, ornamental fish culture temperature can be reduced, such that a purpose for saving energy is achieved.
Description
technical field [0001] The invention relates to a variety improvement technology of economic fish and ornamental fish, in particular to a method for improving the ability of fish to withstand low temperature. Background technique [0002] At present, there are not many methods to improve the low temperature tolerance of fish. Mainly rely on natural individual selection and cross breeding, transgenic methods are also under development. These methods select fish individuals with strong cold resistance through natural mutations, fix the cold resistance characteristics through hybridization, or improve the cold resistance of fish by transferring genes related to cold resistance. However, these methods have disadvantages such as low efficiency, long time or high requirements for technical equipment, and it is difficult to be widely used in some small producers and uncommon fishes. [0003] Through the search of existing patent documents, it is found that the Chinese invention p...
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IPC IPC(8): A01K61/10
CPCA01K61/00Y02A40/81
Inventor 张俊芳韩兵社罗军涛孙宇田
Owner SHANGHAI OCEAN UNIV