Pelophylax nigromaculata breeding method
A kind of technology of black-spotted frog and breeding method, applied in the direction of animal husbandry and other directions, can solve the problems of unfavorable flesh quality of black-spotted frog and increase cost, and achieve the effects of weight gain, volume increase and disease resistance enhancement.
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[0017] The present invention will be further described below in conjunction with embodiment:
[0018] 1 Materials and methods
[0019] 1.1 Experimental equipment
[0020] Artificial climate chamber (10M 2 ), analytical balance (±0.0001g, Sartorius Scientific Instrument Co., Ltd.), biochemical constant temperature incubator, XTS3 dissecting mirror (Beijing Tektronix Instrument Co., Ltd.), plant pulverizer (Tianjin Tester Instrument Co., Ltd.), digital Camera (Sony DSC-T100), digital vernier caliper (±0.01mm), HOBO recording block and software, plastic case (700L×500W×400H mm 3 ), plastic bowl (caliber 20mm), fishing net, petri dish, Morris-type water maze video tracking analysis system (Anhui Zhenghua Biological Instrument Equipment Co., Ltd.), etc.
[0021] 1.2 Collection of experimental animals and hatching of frog eggs
[0022] At night, nearly 15 crayfish of similar size were artificially caught from the persistent breeding pond with a net (mesh 2mm) by flashlight, brou...
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