Detection method and application of lung cancer genes
A detection method, lung cancer technology, applied in the field of genetic detection, can solve the problems of missing gene mutation information, incomplete genes, low sequencing sensitivity and accuracy, etc.
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[0054] The detection method in this case mainly includes extracting free circulating DNA from blood, constructing a cfDNA library, using the designed capture probes of lung cancer-related genes to capture the target region, sequencing on the computer, and finally using bioinformatics to analyze big data. Obtain the mutation information of lung cancer-related genes, such as Figure 6 shown.
[0055] The detection method in this case is mainly composed of three parts, among which a real-time, non-invasive, accurate and comprehensive cfDNA detection technology solution for lung cancer is proposed.
[0056] The first part, cfDNA extraction, purification, library construction.
[0057] The second part is the specific capture of ctDNA lung cancer-related genes.
[0058] The third part is ultra-high-depth sequencing analysis of ctDNA lung cancer gene sequencing with a depth of more than 10,000x, which detects gene variation information related to the occurrence and development of l...
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