Application of synm in preparation of tools for diagnosing or treating endometrial cancer

An endometrial cancer and tool technology, applied in the fields of cancer diagnosis, treatment, and prognosis prediction, can solve the problems of low detection rate, pain caused by patients, and no effective early diagnosis and prognosis indicators for endometrial cancer.

Active Publication Date: 2019-08-23
QINGDAO MEDINTELL BIOMEDICAL CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The diagnosis and clinical staging of endometrial cancer are mainly determined based on the results of medical history, clinical examination, pathological examination and various auxiliary examinations. Currently, the main detection methods include B-ultrasound examination, diagnostic curettage, hysteroscopy and lymphography. The detection rate is Not high, and easy to cause pain to patients
Currently, there are no effective early diagnosis and prognostic indicators for endometrial cancer

Method used

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  • Application of synm in preparation of tools for diagnosing or treating endometrial cancer
  • Application of synm in preparation of tools for diagnosing or treating endometrial cancer
  • Application of synm in preparation of tools for diagnosing or treating endometrial cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0069] Example 1 Gene Chip Screening for Differentially Expressed Genes

[0070] 1. Sample collection:

[0071] Endometrial cancer tissue samples: Patients with endometrial cancer were collected, all of whom underwent surgical treatment, and 10 surgical paraffin specimens were collected. All patients were diagnosed with endometrial cancer by pathological examination. Other enrollment conditions are: all patients have not received any treatment before admission; no other malignant tumors; no other hormone-related diseases; complete clinical data.

[0072] 10 cases of endometrial cancer patients, the average age of onset was 58 years old. The main clinical manifestations of patients are irregular vaginal bleeding, lower abdominal pain, menstrual disorders, vaginal discharge, etc. There are also some patients who have no obvious symptoms and are found in physical examination. The endometrial cancer specimens were sectioned by HE staining, and the histomorphological diagnosis w...

Embodiment 2

[0084] Example 2 Large sample validation of differentially expressed genes screened out

[0085] Considering the gene that has not been studied in the prior art on the correlation between this gene and endometrial cancer as a candidate gene, and considering the results of gene sequencing, select the SYNM gene (its expression is down-regulated in endometrial cancer tissue) for verification .

[0086] 1. Sample collection

[0087] According to the method of Example 1, 50 cases of endometrial cancer tissues and 60 cases of normal endometrial tissues were collected.

[0088] 2. Validation at the mRNA level

[0089] 2.1 Extract tissue RNA

[0090] Step is with embodiment 1.

[0091] 2.2 Reverse transcription

[0092] Using a reverse transcription kit, 1 μg of total RNA was reverse-transcribed with reverse transcription buffer to synthesize cDNA. Use 25μl reaction system, take 1μg total RNA for each sample as template RNA, and add the following components in PCR tubes: DEPC wa...

Embodiment 3

[0115] Example 3 SYNM gene overexpression

[0116] 1. Plasmid construction

[0117] Amplification primers are designed according to the coding sequence of the SYNM gene, and the design of primers is well known to those skilled in the art. Amplify the coding sequence of the full-length SYNM gene from the cDNA library of adult fetal brain (clontech company, article number: 638831), insert the above cDNA sequence into the eukaryotic cell expression vector pcDNA3.1, and connect the obtained recombinant vector pcDNA3.1 -SYNM for subsequent experiments.

[0118] 2. Culture and transfection of endometrial cancer cells

[0119] 2.1 Cell culture

[0120] The Ishikawa3-H-12 cell line was cultured in RPMI 1640 medium (containing 10% fetal bovine serum, 100 U / ml penicillin, 100 g / ml streptomycin) in 50 ml culture flasks, at 37 °C, 5% CO 2 Continuous cultivation in a humidified incubator.

[0121] 2.2 Cell transfection

[0122] 1. The day before transfection, 0.5-2*10 5 Tumor cells ...

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Abstract

The invention belongs to the field of biological medicines and discloses application of SYNM in preparation of tool for diagnosing or treating endometrial cancer. Experiments prove that the expressions of an SYNM gene in a normal endometrial tissue and an endometrial cancer tissue are significantly different, so that the SYNM can be used for developing products for diagnosing the endometrial cancer. The invention further discloses the SYNM gene which can be taken as a treatment target of the endometrial cancer. According to the research results of the invention, theoretical basis can be provided for clinicians to formulate individual-based treatment scheme, and a new drug target can be provided for the development of medicines for the endometrial cancer.

Description

technical field [0001] The present invention relates to the fields of cancer diagnosis, treatment and prognosis prediction. More specifically, the present invention relates to a method for cancer diagnosis and prognosis prediction by means of detecting SYNM abnormality; and a cancer therapeutic agent for activating SYNM gene or protein. Background technique [0002] Endometrial cancer is the most commonly diagnosed malignancy of the female reproductive tract in the United States. Most women with endometrial cancer are treated surgically with hysterectomy; however, about 15% of women demonstrate persistent or recurrent tumors that are refractory to current chemotherapy. For these women with advanced, progressive, or recurrent disease, survival is poor because there are no proven adjuvant treatments. The average survival time after relapse is 10 months, and the 5-year survival rate for patients who have relapsed is only 13%. [0003] The diagnosis and clinical staging of end...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6886G01N33/68G01N33/574A61K45/00A61P35/00
CPCA61K45/00C12Q1/6886C12Q2600/158G01N33/57442G01N33/68
Inventor 杨承刚王晓云宋宏涛
Owner QINGDAO MEDINTELL BIOMEDICAL CO LTD
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