Application of a phenylpropanoid compound in the preparation of medicines for treating inflammatory diseases
A technology for inflammatory diseases and compounds, applied in the field of medicine, can solve problems such as complex biological effects, achieve high purity, simple extraction and separation methods, and easy synthesis
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Embodiment 1
[0049] Example 1 Preparation of phenylpropanoid compounds
[0050] The present embodiment provides a kind of preparation method of phenylpropanoid compound shown in formula (I), comprises the following steps:
[0051] S1. Take 50kg of large leaves, use the roots as raw materials, dry them, and cut them into small pieces. After 8 times of 60% ethanol reflux extraction for 3 times, each time for 2 hours, the extracts were combined, concentrated until there was no alcohol smell, and the extract was ready for use;
[0052] S2. Dissolve the concentrated extract in step S1 in 10 L of water, use D101 macroporous adsorption resin column to elute it, the eluent is water, elute 3 column volumes, collect the eluent, and name it as MM -1, spare;
[0053] S3. The fraction MM-1 collected in step S2 is eluted with reverse-phase ODS column chromatography, the eluent is methanol-water system, and its volume ratio is 25:75, eluting 18 column volumes, press Collect a fraction of the eluent ev...
Embodiment 2
[0056] Example 2 Preparation of phenylpropanoid compounds
[0057] The present embodiment provides a kind of preparation method of phenylpropanoid compound shown in formula (I), comprises the following steps:
[0058] S1. Take 40kg of large leaves, use the roots as raw materials, dry them, and cut them into small pieces. After 6 times of 50% ethanol reflux extraction for 2 times, each time for 1 hour, the extracts were combined, concentrated until there was no alcohol smell, and the extract was used for later use;
[0059] S2. Dissolve the concentrated extract in step S1 in 5L of water, use D101 macroporous adsorption resin column to elute it, the eluent is that the volume ratio of ethanol and water is 15:85, and the elution is carried out for 3 column volumes , the eluate was collected and named as MM-1 for use;
[0060] S3. The fraction MM-1 collected in step S2 is eluted with reverse-phase ODS column chromatography, the eluent is methanol-water system, and its volume rati...
Embodiment 3
[0063] Example 3 Preparation of phenylpropanoid compounds
[0064] The present embodiment provides a kind of preparation method of phenylpropanoid compound shown in formula (I), comprises the following steps:
[0065] S1. Take 60kg of large leaves, use the roots as raw materials, dry them, and cut them into small pieces. 7 times the amount of 70% ethanol reflux extraction for 4 times, each time for 3 hours, the extracts were combined, concentrated until there was no alcohol smell, and the extract was used for later use;
[0066] S2. Dissolve the concentrated extract in step S1 in 8L of water, use D101 macroporous adsorption resin column to elute it, the eluent is that the volume ratio of ethanol and water is 10:90, and the elution is carried out for 3 column volumes , the eluate was collected and named as MM-1 for use;
[0067] S3. The fraction MM-1 collected in step S2 is eluted with reverse-phase ODS column chromatography, the eluent is methanol-water system, and its volum...
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