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Breast cancer diagnosis kit adopting glutathion peroxidase 3 as marker

A technology of glutathione peroxide and a kit is applied in the field of protein detection to achieve the effects of convenient operation, low cost and good accuracy

Inactive Publication Date: 2016-11-09
XIAMEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Using GSH-Px3 as a diagnostic marker for breast cancer has not been reported in this field

Method used

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  • Breast cancer diagnosis kit adopting glutathion peroxidase 3 as marker
  • Breast cancer diagnosis kit adopting glutathion peroxidase 3 as marker

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1, GSH-Px3 can be used as expression verification of breast cancer diagnostic markers

[0036] Using Western blotting method, 12% polyacrylamide gel was used, and 15ug of each serum sample was loaded. The primary antibody of GSH-Px3 was: glutathione peroxidase 3 mouse monoclonal antibody, which Dilute to 0.2μg / ml-0.5μg / ml with PBS buffer, preferably 0.2μg / ml; the secondary antibody used is: peroxidase goat anti-mouse IgG polyclonal antibody, which is diluted to 0.01μg / ml with PBS buffer ml - 0.02 μg / ml, preferably 0.02 μg / ml. from figure 1 and figure 2 In the study, it was found that the concentration of GSH-Px3 in the serum of breast cancer patients was higher than that of healthy controls, and the significance was analyzed by the expression of GSH-Px3 in serum between healthy people and breast cancer patients (T-text ), if the expression level of GSH-Px3 in the blood sample to be tested is compared with the expression level of GSH-Px3 in the serum of healt...

Embodiment 2

[0039] Embodiment 2, breast cancer diagnosis kit of the present invention

[0040] This embodiment provides a kit for breast cancer diagnosis, including an antibody that can bind, adsorb and / or couple to GSH-Px3.

[0041] Specifically, the antibodies include: glutathione peroxidase 3 primary antibody, specifically glutathione peroxidase 3 mouse monoclonal antibody, the advantages of using it as a primary antibody are: high sensitivity, specificity It has good sex and can detect glutathione peroxidase in human serum 3), the antibody also includes: a secondary antibody that recognizes the primary antibody, specifically a peroxidase goat anti-mouse IgG polyclonal antibody; the advantage is sensitivity High and specific, can bind glutathione peroxidase 3 primary antibody.

[0042] Preferably, the working concentration range of the primary antibody: 0.2 μg / ml-0.5 μg / ml; the working concentration range of the secondary antibody: 0.01 μg / ml-0.02 μg / ml.

[0043] Further, the kit als...

Embodiment 3

[0045] Embodiment 3, adopt kit described in the present invention to carry out breast cancer diagnosis

[0046] In the present embodiment, 11 blood samples of breast cancer patients were collected, and the kit of the present invention was used to diagnose breast cancer, and 9 of them were diagnosed with breast cancer, with a detection rate of 88.2%; 20 blood samples of healthy people were detected, GSH-Px3 content was normal.

[0047] Among the existing serological diagnostic methods for breast cancer in this field, the most commonly used detection method is the detection method (enzyme-linked immunosorbent assay) using CEA as a tumor marker. This method was used to detect the same 11 breast cancer patient blood samples above, and the results were Eight were found to have breast cancer.

[0048] The matching rate of the kit of the present invention and the conventional method is 89.9%.

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Abstract

The invention relates to a breast cancer diagnosis kit adopting glutathion peroxidase 3 as a marker, and belongs to the field of protein detection. The kit is characterized in that the glutathion peroxidase 3 is adopted as the marker. The breast cancer diagnosis kit comprises an antibody which can bind, adsorb and / or couple with the glutathion peroxidase 3. The kit has the advantages of convenience in operation, low cost, high sensitivity and good accuracy when breast cancer diagnosis is carried out by adopting the GSH-Px3 as the marker and / or adopting the kit.

Description

technical field [0001] The invention belongs to the field of protein detection, in particular to a breast cancer diagnostic kit using glutathione peroxidase 3 as a marker. Background technique [0002] The female mammary gland is composed of skin, fibrous tissue, mammary glands and fat. Breast cancer is a malignant tumor that occurs in the mammary gland epithelial tissue. The global incidence of breast cancer has been on the rise since the late 1970s. 1 in 8 women in the U.S. will develop breast cancer in her lifetime. China is not a country with a high incidence of breast cancer, but it should not be optimistic. In recent years, the growth rate of the incidence of breast cancer in my country is 1 to 2 percentage points higher than that of countries with a high incidence. According to the 2009 breast cancer incidence data released by the National Cancer Center and the Ministry of Health's Bureau of Disease Control and Prevention in 2012, the incidence of breast cancer in t...

Claims

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Application Information

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IPC IPC(8): G01N33/574G01N33/531
CPCG01N33/57415G01N33/531G01N33/57488
Inventor 王团老陈婉明洪万进
Owner XIAMEN UNIV